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Significance of phosphoenolpyruvate carboxylase during ammonium assimilation: carbon isotope discrimination in photosynthesis and respiration by the N-limited green alga Selenastrum minutum

The effect of N-assimilation on the partitioning of carbon fixation between phosphoenolpyruvate carboxylase (PEPcase) and ribulose bisphosphate carboxylase/oxygenase (Rubisco) was determined by measuring stable carbon isotope discrimination during photosynthesis by an N-limited green alga, Selenastr...

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Published in:	Plant physiology (Bethesda) 1989-04, Vol.89 (4), p.1150-1157
Main Authors:	Guy, R.D. (Queen's University, Kingston, Ontario, Canada), Vanlerberghe, G.C, Turpin, D.H
Format:	Article
Language:	English
Subjects:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ALGAE ALGUE Biological and medical sciences Carbon cycle Carbon dioxide Carbon isotopes CARBONE CARBONO COMPOSE DE L'AMMONIUM COMPUESTOS DE AMONIO Freshwater Fundamental and applied biological sciences. Psychology Irradiance Isotope effects LIASAS LYASE Metabolism METABOLISME DE L'AZOTE METABOLISMO DEL NITROGENO Photosynthesis Photosynthesis, respiration. Anabolism, catabolism Plant physiology and development Plants Quaternary ammonium compounds RESPIRACION RESPIRATION Selenastrum minutum VIA BIOQUIMICA DEL METABOLISMO VOIE BIOCHIMIQUE DU METABOLISME
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container_title	Plant physiology (Bethesda)
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creator	Guy, R.D. (Queen's University, Kingston, Ontario, Canada) Vanlerberghe, G.C Turpin, D.H
description	The effect of N-assimilation on the partitioning of carbon fixation between phosphoenolpyruvate carboxylase (PEPcase) and ribulose bisphosphate carboxylase/oxygenase (Rubisco) was determined by measuring stable carbon isotope discrimination during photosynthesis by an N-limited green alga, Selenastrum minutum (Naeg.) Collins. This was facilitated by a two process model accounting for simultaneous CO2 fixation and respiratory CO2 release. Discrimination by control cells was consistent with the majority of carbon being fixed by Rubisco. During nitrogen assimilation however, discrimination was greatly reduced indicating an enhanced flux through PEPcase which accounted for upward of 70% of total carbon fixation. This shift toward anaplerotic metabolism supports a large increase in tricarboxylic acid cycle activity primarily between oxaloacetate and alpha-ketoglutarate thereby facilitating the provision of carbon skeletons for amino acid synthesis. This provides an example of a unique set of conditions under which anaplerotic carbon fixation by PEPcase exceeds photosynthetic carbon fixation by Rubisco in a C3 organism
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Anabolism, catabolism ; Plant physiology and development ; Plants ; Quaternary ammonium compounds ; RESPIRACION ; RESPIRATION ; Selenastrum minutum ; VIA BIOQUIMICA DEL METABOLISMO ; VOIE BIOCHIMIQUE DU METABOLISME</subject><ispartof>Plant physiology (Bethesda), 1989-04, Vol.89 (4), p.1150-1157</ispartof><rights>Copyright 1989 American Society of Plant Physiologists</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4271979$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4271979$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19429862$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16666678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guy, R.D. (Queen's University, Kingston, Ontario, Canada)</creatorcontrib><creatorcontrib>Vanlerberghe, G.C</creatorcontrib><creatorcontrib>Turpin, D.H</creatorcontrib><title>Significance of phosphoenolpyruvate carboxylase during ammonium assimilation: carbon isotope discrimination in photosynthesis and respiration by the N-limited green alga Selenastrum minutum</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The effect of N-assimilation on the partitioning of carbon fixation between phosphoenolpyruvate carboxylase (PEPcase) and ribulose bisphosphate carboxylase/oxygenase (Rubisco) was determined by measuring stable carbon isotope discrimination during photosynthesis by an N-limited green alga, Selenastrum minutum (Naeg.) Collins. This was facilitated by a two process model accounting for simultaneous CO2 fixation and respiratory CO2 release. Discrimination by control cells was consistent with the majority of carbon being fixed by Rubisco. During nitrogen assimilation however, discrimination was greatly reduced indicating an enhanced flux through PEPcase which accounted for upward of 70% of total carbon fixation. This shift toward anaplerotic metabolism supports a large increase in tricarboxylic acid cycle activity primarily between oxaloacetate and alpha-ketoglutarate thereby facilitating the provision of carbon skeletons for amino acid synthesis. This provides an example of a unique set of conditions under which anaplerotic carbon fixation by PEPcase exceeds photosynthetic carbon fixation by Rubisco in a C3 organism</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ALGAE</subject><subject>ALGUE</subject><subject>Biological and medical sciences</subject><subject>Carbon cycle</subject><subject>Carbon dioxide</subject><subject>Carbon isotopes</subject><subject>CARBONE</subject><subject>CARBONO</subject><subject>COMPOSE DE L'AMMONIUM</subject><subject>COMPUESTOS DE AMONIO</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Irradiance</subject><subject>Isotope effects</subject><subject>LIASAS</subject><subject>LYASE</subject><subject>Metabolism</subject><subject>METABOLISME DE L'AZOTE</subject><subject>METABOLISMO DEL NITROGENO</subject><subject>Photosynthesis</subject><subject>Photosynthesis, respiration. Anabolism, catabolism</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Quaternary ammonium compounds</subject><subject>RESPIRACION</subject><subject>RESPIRATION</subject><subject>Selenastrum minutum</subject><subject>VIA BIOQUIMICA DEL METABOLISMO</subject><subject>VOIE BIOCHIMIQUE DU METABOLISME</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNp9kU2PFCEQhonRuOPqzZMxhovrqUfohgH2ZjZ-JRs9zHru1DR0LxsaWqA39o_zv8nYo94kEKi8T70VqhB6TsmWUsLeTtNWqi0rAScP0Ibypq5qzuRDtCGkvImU6gw9SemOEEIbyh6jM7o7LiE36OfeDt72tgPfGRx6PN2GVI7xwU1LnO8hG9xBPIQfi4NksJ6j9QOGcQzeziOGlOxoHWQb_OVKemxTyGEqsE1dLLL_LWPrj_Y5pMXnW5NswuA1jiZNNq7EYcFFwV8qV7Ky0XiIxngMbgC8N854SDmWqsVyzvP4FD3qwSXz7HSfo5sP72-uPlXXXz9-vnp3XfW1ELmSmoiewU6AFppIQTpFGkI511p0sgSkoR2rOYeekp0EKc2xPYaLvtN615yjN6vtFMP32aTcjuVjxjnwJsypFU3DVMNoXciL_5KUSykUlwV8dQLnw2h0O5U2QVzaP5MpwOsTAKkD18cyIJv-cYrVSu6OFV-u3F3KIf7VWS2oEqrIL1a5h9DCEIvFt70itGzR_ALWrrRK</recordid><startdate>19890401</startdate><enddate>19890401</enddate><creator>Guy, R.D. 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Psychology</topic><topic>Irradiance</topic><topic>Isotope effects</topic><topic>LIASAS</topic><topic>LYASE</topic><topic>Metabolism</topic><topic>METABOLISME DE L'AZOTE</topic><topic>METABOLISMO DEL NITROGENO</topic><topic>Photosynthesis</topic><topic>Photosynthesis, respiration. Anabolism, catabolism</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Quaternary ammonium compounds</topic><topic>RESPIRACION</topic><topic>RESPIRATION</topic><topic>Selenastrum minutum</topic><topic>VIA BIOQUIMICA DEL METABOLISMO</topic><topic>VOIE BIOCHIMIQUE DU METABOLISME</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guy, R.D. (Queen's University, Kingston, Ontario, Canada)</creatorcontrib><creatorcontrib>Vanlerberghe, G.C</creatorcontrib><creatorcontrib>Turpin, D.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guy, R.D. (Queen's University, Kingston, Ontario, Canada)</au><au>Vanlerberghe, G.C</au><au>Turpin, D.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Significance of phosphoenolpyruvate carboxylase during ammonium assimilation: carbon isotope discrimination in photosynthesis and respiration by the N-limited green alga Selenastrum minutum</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1989-04-01</date><risdate>1989</risdate><volume>89</volume><issue>4</issue><spage>1150</spage><epage>1157</epage><pages>1150-1157</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The effect of N-assimilation on the partitioning of carbon fixation between phosphoenolpyruvate carboxylase (PEPcase) and ribulose bisphosphate carboxylase/oxygenase (Rubisco) was determined by measuring stable carbon isotope discrimination during photosynthesis by an N-limited green alga, Selenastrum minutum (Naeg.) Collins. This was facilitated by a two process model accounting for simultaneous CO2 fixation and respiratory CO2 release. Discrimination by control cells was consistent with the majority of carbon being fixed by Rubisco. During nitrogen assimilation however, discrimination was greatly reduced indicating an enhanced flux through PEPcase which accounted for upward of 70% of total carbon fixation. This shift toward anaplerotic metabolism supports a large increase in tricarboxylic acid cycle activity primarily between oxaloacetate and alpha-ketoglutarate thereby facilitating the provision of carbon skeletons for amino acid synthesis. This provides an example of a unique set of conditions under which anaplerotic carbon fixation by PEPcase exceeds photosynthetic carbon fixation by Rubisco in a C3 organism</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16666678</pmid><doi>10.1104/pp.89.4.1150</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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subjects	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ALGAE ALGUE Biological and medical sciences Carbon cycle Carbon dioxide Carbon isotopes CARBONE CARBONO COMPOSE DE L'AMMONIUM COMPUESTOS DE AMONIO Freshwater Fundamental and applied biological sciences. Psychology Irradiance Isotope effects LIASAS LYASE Metabolism METABOLISME DE L'AZOTE METABOLISMO DEL NITROGENO Photosynthesis Photosynthesis, respiration. Anabolism, catabolism Plant physiology and development Plants Quaternary ammonium compounds RESPIRACION RESPIRATION Selenastrum minutum VIA BIOQUIMICA DEL METABOLISMO VOIE BIOCHIMIQUE DU METABOLISME
title	Significance of phosphoenolpyruvate carboxylase during ammonium assimilation: carbon isotope discrimination in photosynthesis and respiration by the N-limited green alga Selenastrum minutum
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