Characterization of the expression of the petunia glycine-rich protein-1 gene product

We have examined the expression of the petunia (Petunia hybrida) glycine-rich protein-1 (ptGRP1) gene product using an antibody raised against a synthetic peptide comprising amino acids 22 through 36 of the mature ptGRP1 protein. This antibody recognizes a single protein of 23 kilodaltons. Cell frac...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1990-06, Vol.93 (2), p.596-602
Main Authors: Condit, C.M. (University of California-Berkeley, Berkeley, CA), McLean, B.G, Meagher, R.B
Format: Article
Language:English
Subjects:
Amino acids
Antibodies
Biological and medical sciences
buds
Cell walls
CODE GENETIQUE
CODIGO GENETICO
Developmental biology
Epidermal cells
ETAPAS DE DESARROLLO DE LA PLANTA
Fundamental and applied biological sciences. Psychology
GENE
Gene expression
GENES
GLICINA
GLYCINE
Leaves
Molecular and cellular biology
Molecular Biology and Gene Regulation
Molecular genetics
PETUNIA
Plant antigens
Plants
SINTESIS DE PROTEINAS
Soybeans
STADE DE DEVELOPPEMENT VEGETAL
SYNTHESE PROTEIQUE
Vascular tissues
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have examined the expression of the petunia (Petunia hybrida) glycine-rich protein-1 (ptGRP1) gene product using an antibody raised against a synthetic peptide comprising amino acids 22 through 36 of the mature ptGRP1 protein. This antibody recognizes a single protein of 23 kilodaltons. Cell fractionation studies showed that, as predicted (CM Condit, RB Meagher [1986] Nature 323: 178-181), ptGRP1 is most likely localized in the cell wall. In addition, it was found that (extractable) ptGRP1 is present in much higher abundance in unexpanded than in fully expanded tissue, with highest levels of accumulation in the bud. This same developmentally regulated pattern of protein expression was found in all varieties of petunia tested. In addition, tissue blots of petunia stem sections showed that ptGRP1 is localized to within the vascular tissue (to at least the phloem or cambium) and to either the epidermal cells or to a layer of collenchyma cells directly below the epidermis. Localization of ptGRP1 antigen in these cell types is shown to occur at different times in the overall development of the plant and at different quantitative levels
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.93.2.596