Molecular cloning and relationship to freezing tolerance of cold-acclimation-specific genes of alfalfa

Cold-acclimation-specific (CAS) gene expression has been examined by screening a cDNA library prepared from poly(A)+ RNA of cold-acclimated seedlings of a freezing-tolerant variety of alfalfa (Medicago falcata cv Anik). Three CAS cDNA clones, pSM784, pSM2201, and pSM2358, representing different sequ...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1989-01, Vol.89 (1), p.375-380
Main Authors: Mohapatra, S.S, Wolfraim, L, Poole, R.J, Dhindsa, R.S
Format: Article
Language:English
Subjects:
Acclimatization
ADAPTACION FISIOLOGICA
ADAPTATION
ADN
Alfalfa
amino acid sequence
Biological and medical sciences
cDNA
CLONACION
CLONAGE
CLONING
COLD
cold-acclimation-specific gene
Complementary DNA
DNA
Freezing
FRIO
FROID
Fundamental and applied biological sciences. Psychology
GENE
gene expression
GENES
Genes. Genome
Medicago falcata cv. Anik
MEDICAGO SATIVA
Messenger RNA
Molecular and cellular biology
Molecular genetics
nucleotide sequence
Plants
predictions
RESISTANCE A LA TEMPERATURE
RESISTENCIA A LA TEMPERATURA
RNA
Seedlings
TEMPERATURE RESISTANCE
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Summary:Cold-acclimation-specific (CAS) gene expression has been examined by screening a cDNA library prepared from poly(A)+ RNA of cold-acclimated seedlings of a freezing-tolerant variety of alfalfa (Medicago falcata cv Anik). Three CAS cDNA clones, pSM784, pSM2201, and pSM2358, representing different sequence species, have been used to investigate the relative abundance and time-course of accumulation of corresponding transcripts. Results obtained show that the expression of these CAS genes is regulated in a coordinated manner most likely at the level of transcription. The expression of genes, as measured by mRNA abundance corresponding to the three CAS cDNA clones, is not stimulated or induced by heat shock, water stress, abscisic acid, or wounding. A positive correlation is observed between the expression of these cloned sequences and the degree of freezing-tolerance in four alfalfa cultivars.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.89.1.375