Fructosyl transfer between 1-kestose and sucrose in wheat leaves

The labeling pattern of the sugar moieties of 1-kestose after in vivo pulse labeling with 14CO2 was not the same as that after in vitro labeling with 14C-sucrose. The two fructosyl residues of 1-kestose had similar specific radioactivities after in vitro synthesis, but after in vivo radiolabeling th...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1991-05, Vol.96 (1), p.251-254
Main Authors: Kanabus, J. (Purdue University, West Lafayette, IN), Gibeaut, D.M, Carpita, N.C, Housley, T.L
Format: Article
Language:English
Subjects:
Artichokes
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
FEUILLE
Fructans
Fundamental and applied biological sciences. Psychology
HOJAS
Leaves
Metabolism
METABOLISME
METABOLISMO
Oligomers
OLIGOSACARIDOS
OLIGOSACCHARIDE
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
Plants
POLISACARIDOS
POLYHOLOSIDE
Product labeling
Radioactive decay
SACCHAROSE
SUCROSA
TECHNIQUE DES TRACEURS
TECNICAS DE TRAZADORES
Trisaccharides
TRITICUM AESTIVUM
Tubers
Vacuoles
VIA BIOQUIMICA DEL METABOLISMO
VOIE BIOCHIMIQUE DU METABOLISME
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Summary:The labeling pattern of the sugar moieties of 1-kestose after in vivo pulse labeling with 14CO2 was not the same as that after in vitro labeling with 14C-sucrose. The two fructosyl residues of 1-kestose had similar specific radioactivities after in vitro synthesis, but after in vivo radiolabeling the specific radioactivity of the terminal fructosyl moiety was significantly less than the internal fructosyl moiety. Evidence is presented that the uneven specific radioactivity of in vivo radiolabeling results from enzymatic transfer of terminal fructosyl residue from 1-kestose to sucrose
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.96.1.251