Organ-specific and environmentally regulated expression of two abscisic acid-induced genes of tomato. Nucleotide sequence and analysis of the corresponding cDNAs

The cDNAs, pLE4 and pLE25, represent mRNAs that accumulate in response to water deficit and elevated levels of endogenous abscisic acid in detached leaves of drought-stressed tomato (Lycopersicon esculentum Mill., cv Ailsa Craig) (A Cohen, EA Bray [1990] Planta 182: 27-33). DNA sequence analysis of...

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Published in:Plant physiology (Bethesda) 1991-12, Vol.97 (4), p.1367-1374
Main Authors: Cohen, A. (University of California, Riverside, CA), Plant, A.L, Moses, M.S, Bray, E.A
Format: Article
Language:English
Subjects:
abscisic acid
ACIDE ABSCISSIQUE
ACIDO ABSCISICO
ADN
Amino acids
Biological and medical sciences
CODE GENETIQUE
CODIGO GENETICO
Complementary DNA
ESTRES DE SEQUIA
EXPRESION GENICA
EXPRESSION DES GENES
Fruits
Fundamental and applied biological sciences. Psychology
Gene expression
Gene expression regulation
genes
GENETICA
GENETIQUE
Leaves
LYCOPERSICON ESCULENTUM
Messenger RNA
Molecular and cellular biology
Molecular genetics
NUCLEOTIDE
NUCLEOTIDOS
Plant roots
Plants
RNA
SECHERESSE
Seeds
SEQUIA
STRESS DU A LA SECHERESSE
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Summary:The cDNAs, pLE4 and pLE25, represent mRNAs that accumulate in response to water deficit and elevated levels of endogenous abscisic acid in detached leaves of drought-stressed tomato (Lycopersicon esculentum Mill., cv Ailsa Craig) (A Cohen, EA Bray [1990] Planta 182: 27-33). DNA sequence analysis of pLE4 and pLE25 showed that the deduced polypeptides were 13.9 and 9.3 kilodaltons, respectively. Each polypeptide was hydrophilic, cysteine- and tryptophan-free, and found to be similar to previously identified proteins that accumulate during the late stages of embryogenesis. pLE4 and pLE25 mRNA accumulated in a similar organ-specific pattern in response to specific abiotic stresses. Yet, expression patterns of the corresponding genes in response to developmental cues were not similar. pLE25 mRNA accumulated to much higher levels in developing seeds than in drought-stressed vegetative organs. pLE4 mRNA accumulated predominantly in drought-stressed leaves. The similarities and differences in the accumulation characteristics of these two mRNAs indicates that more than one mechanism exists for the regulation of their corresponding genes
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.97.4.1367