Enzymatic synthesis of isoprene from dimethylallyl diphosphate in Aspen leaf extracts

Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl,1-3-butadiene). This isoprene synthase activity has been part...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1991-12, Vol.97 (4), p.1588-1591
Main Authors: Silver, G.M. (University of Colorado, Boulder, CO), Fall, R
Format: Article
Language:English
Subjects:
ACTIVIDAD CATALITICA
ACTIVIDAD ENZIMATICA
ACTIVITE CATALYTIQUE
ACTIVITE ENZYMATIQUE
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
Biosynthesis
Chloroplasts
Communications
Diphosphates
enzymatic activity
Enzyme activity
Enzymes
ESTER
ESTERES
EXTRACTOS VEGETALES
extracts
EXTRAIT D'ORIGINE VEGETALE
FEUILLE
FOSFATOS
Fundamental and applied biological sciences. Psychology
HOJAS
identification
isoprene synthase
leaves
LIPIDE
LIPIDOS
Metabolism
Metabolism. Physicochemical requirements
PHOSPHATE
Phosphates
Plant biochemistry
Plant physiology and development
Plants
POPULUS TREMULOIDES
Terpenoids
VIA BIOQUIMICA DEL METABOLISMO
VOIE BIOCHIMIQUE DU METABOLISME
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Summary:Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl,1-3-butadiene). This isoprene synthase activity has been partially purified. The nonenzymatic reaction of dimethylallyl diphosphate to isoprene, known to be acid catalyzed, may be insignificant at physiological pH. In contrast, the enzymatic reaction may be responsible for the majority of light-dependent isoprene production by isoprene-emitting plants
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.97.4.1588