Efficiency of particle-bombardment-mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco

Plasmid DNA pBl221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G2 phases gave 4 to 6 times higher transformation efficiency th...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1991-12, Vol.97 (4), p.1585-1587
Main Authors: Iida, A. (Sumitomo Chemical Co., Ltd., Hyogo, Japan), Yamashita, T, Yamada, Y, Morikawa, H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cell aggregates
cell culture
Cell culture techniques
Cell cycle
Communications
Cultured cells
ETAPAS DE DESARROLLO DE LA PLANTA
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Guns
Japanese culture
Methods. Procedures. Technologies
Mitotic index
NICOTIANA TABACUM
Plant cells
Plasmids
Pneumatics
STADE DE DEVELOPPEMENT VEGETAL
synchronization
transfer
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSFORMACION GENETICA
transformation
TRANSFORMATION GENETIQUE
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Summary:Plasmid DNA pBl221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G2 phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G1 phases
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.97.4.1585