Light and Fungal Elicitor Induce 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase mRNA in Suspension Cultured Cells of Parsley (Petroselinum crispum L.)

Light and fungal elicitor induce mRNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase in suspension cultured cells of parsley (Petroselinum crispum L.). The kinetics and dose response of mRNA accumulation were similar for DAHP synthase and phenylalanine ammonia-lyase (PAL). Six...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-02, Vol.98 (2), p.761-763
Main Authors: Henstrand, John M., McCue, Kent F., Kent Brink, Handa, Avtar K., Herrmann, Klaus M., Conn, Eric E.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biosynthesis
Cell culture techniques
Communications
Cultured cells
Enzymes
Fundamental and applied biological sciences. Psychology
Gene expression regulation
Genes
Kinetics
Messenger RNA
Metabolism
Molecular Biology and Gene Regulation
Nitrogen metabolism
Parsley
Plant physiology and development
Shikimate pathway
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Summary:Light and fungal elicitor induce mRNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase in suspension cultured cells of parsley (Petroselinum crispum L.). The kinetics and dose response of mRNA accumulation were similar for DAHP synthase and phenylalanine ammonia-lyase (PAL). Six micrograms of elicitor from Phytophthora megasperma f. glycinia gave a detectable induction within 1 hour. Induction of DAHP synthase and PAL mRNAs by light was transient, reaching maximal levels at 4 hours and returning to pretreatment levels after 24 hours. Our data suggest that either light or fungal elicitor transcriptionally activate DAHP synthase. A coordinate regulation for key enzymes in the synthesis of primary and secondary metabolites is indicated.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.98.2.761