Replication of Pea Enation Mosaic Virus RNA in Isolated Pea Nuclei

Isolated nuclei from healthy pea plants were primed with pea enation mosaic virus (PEMV), southern bean mosaic virus (SBMV), radish mosaic virus (RdMV), tobacco mosaic virus (TMV), PEMV RNA, SBMV RNA, RdMV RNA, or TMV RNA. RNA replication occurred only with PEMV RNA and not with intact PEMV or any o...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1977-07, Vol.74 (7), p.2919-2922
Main Authors: Powell, C. A., De Zoeten, G. A.
Format: Article
Language:English
Subjects:
Bentonite
Biological Sciences: Cell Biology
Mosaic viruses
Nucleic acids
Peas
Phenols
Plant viruses
RNA
Viral RNA
Virology
Viruses
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Description
Summary:Isolated nuclei from healthy pea plants were primed with pea enation mosaic virus (PEMV), southern bean mosaic virus (SBMV), radish mosaic virus (RdMV), tobacco mosaic virus (TMV), PEMV RNA, SBMV RNA, RdMV RNA, or TMV RNA. RNA replication occurred only with PEMV RNA and not with intact PEMV or any of the other viruses or RNAs, as judged by ensuing actinomycin D-insensitive polymerase activity. Molecular hybridization experiments showed that some of the product of the polymerase was PEMV-specific (-)RNA. The substrate and ionic requirements of this polymerase were the same as those for the RNA-dependent RNA polymerase present in nuclei isolated from PEMV-infected pea plants. No virus particles could be recovered from nuclei primed with PEMV RNA. These results are discussed in relation to the possible mechanism for in vivo infection of pea cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.74.7.2919