Shoot production in squash (Cucurbita pepo) by in vitro organogenesis

Seedling-derived cotyledon explants of squash ( Cucurbita pepo L.) of commercial cultivars True French, Ma'yan and Goldy were regenerated in vitro on Murashige and Skoog medium augmented with 1 mg/l benzyladenine. After 4 weeks in culture small shoots and buds regenerated only on the most proxi...

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Bibliographic Details
Published in:Plant cell reports 2003-04, Vol.21 (8), p.739-746
Main Authors: ANANTHAKRISHNAN, G, XIA, X, ELMAN, C, SINGER, S, PARIS, H. S, GAL-ON, A, GABA, V
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - pharmacology
Benzyl Compounds
Biological and medical sciences
Cotyledon - drug effects
Cotyledon - physiology
Cotyledon - ultrastructure
Cucurbita - drug effects
Cucurbita - genetics
Cucurbita - physiology
Cultivars
Culture Techniques - methods
Darkness
Fundamental and applied biological sciences. Psychology
Gibberellins - pharmacology
Kinetin
Light
Microscopy, Electron, Scanning
Plant Growth Regulators - pharmacology
Plant Shoots - drug effects
Plant Shoots - physiology
Plant Shoots - ultrastructure
Ploidies
Purines
Regeneration - genetics
Seedlings
Shoots
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Summary:Seedling-derived cotyledon explants of squash ( Cucurbita pepo L.) of commercial cultivars True French, Ma'yan and Goldy were regenerated in vitro on Murashige and Skoog medium augmented with 1 mg/l benzyladenine. After 4 weeks in culture small shoots and buds regenerated only on the most proximal cotyledon edge. Culture on an elongation medium with a reduced cytokinin concentration (0.1 mg/l) with or without 1 mg/l gibberellic acid (GA(3)) facilitated the recovery of shoots. Fresh shoots could be recovered at each subculture of the regenerating mass. Peak productivity was during the third cycle of subculture, and shoot production ceased after the fifth subculture. Culture on elongation medium supplemented with GA(3) was 55% more effective with respect to overall shoot production than that on medium without GA(3), with 22 shoots recovered in total per explant from the former. Regeneration occurred under both light and dark conditions. All of the shoots tested were diploid. The shoots were rooted and transferred to the greenhouse where they grew and flowered normally.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-003-0584-y