Cotton Lea4 (D19) and LeaA2 (D132) group 1 Lea genes encoding water stress-related proteins containing a 20-amino acid motif

• Published in: Plant physiology (Bethesda) 1992-06, Vol.99 (2), p.783-788
• Main Authors: Galau, G.A. (University of Georgia, Athens, GA), Wang, H.Y.C, Hughes, D.W
• Format: Article
• Language: English
• Subjects: ACIDE AMINE; ALLELES; AMINO ACID SEQUENCES; AMINO ACIDS; AMINOACIDOS; Biological and medical sciences; CODE GENETIQUE; CODIGO GENETICO; Complementary DNA; DROUGHT STRESS; Embryogenesis; Embryos; ESTRES DE SEQUIA; Fundamental and applied biological sciences. Psychology; GENBANK M38053; GENBANK M73751; GENBANK M73752; GENBANK M83303; GENBANK M83304; GENBANK M85261; GENBANK M86753; GENBANK M86754; GENE; GENE MAPPING; GENES; Genes. Genome; GENETIC CODE; Genomes; GOSSYPIUM HIRSUTUM; Messenger RNA; Molecular and cellular biology; Molecular Biology and Gene Regulation; Molecular genetics; MOLECULAR SEQUENCE DATA; NUCLEOTIDE SEQUENCE; Nucleotides; Plant Gene Register; PLANT PROTEIN; PROTEIN SYNTHESIS; PROTEINAS VEGETALES; PROTEINE VEGETALE; Proteins; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; SINTESIS DE PROTEINAS; STRESS DU A LA SECHERESSE; SYNTHESE PROTEIQUE
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.99.2.783

LEA proteins are a diverse group of probable desiccation protectants that are developmentally induced during the post-abscission stage of late embryo development by ovule abscission and are environmentally induced, probably by a different mechanism(s), in cultured embryos by ABA, desiccation, and osmotic stress. Of the 18 Lea and LeaA mRNAs cloned from cotton, mRNAs of the two related genes Lea4 (cDNA clone D19) and LeaA2 (cDNA clone D132) have the highest cross-hybridization with embryo RNAs of other plants. The cotton LeaA cDNA D19 and the upstream portion of a cotton Lea4 gene have been sequenced. The amino acid sequence of cDNA D19 is peculiar in having a carboxy-terminal extension of 18 amino acids not found in its homologs, but the upstream region of the Lea4 gene does contain elements similar to those defined in a wheat Em gene, the only other group 1 Lea gene sequenced. To confirm the peculiar carboxy extension and to help define the developmental and environmental responsive regions in the cotton Lea4 gene, both alloallels of cotton Lea4 and 10 Lea4 cDNAs were sequenced. The principal results are that there is no carboxy extension in the protein and that transcriptionally important sequences are probably within 268 nucleotides of the transcription start.