Urease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants

Mutation at either of two genetic loci (Eu2 or Eu3) in soybean (Glycine max [L.] Merr.) results in a pleiotropic elimination of the activity of both major urease isozymes. Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-03, Vol.98 (3), p.942-948
Main Authors: Holland, M.A. (University of Missouri, Columbia, MO), Polacco, J.C
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
BACTERIA
Bacteriology
Biological and medical sciences
Callus
FENOTIPOS
FEUILLE
Fundamental and applied biological sciences. Psychology
Genetic mutation
Genotypes
GLYCINE MAX
HOJAS
Leaves
Metabolism. Enzymes
METABOLISME
METABOLISMO
Microbiology
MUTANT
MUTANTES
NICKEL
NIQUEL
OXIDORREDUCTASAS
OXYDOREDUCTASE
PHENOTYPE
Phenotypes
Plants
Scions
Seedlings
Soybeans
UREASA
UREASE
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Summary:Mutation at either of two genetic loci (Eu2 or Eu3) in soybean (Glycine max [L.] Merr.) results in a pleiotropic elimination of the activity of both major urease isozymes. Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1/eu3-e1 mutants is also affected by these plant mutations. The bacteria isolated from leaves of these soybean mutants have transient urease- and hydrogenase-deficient phenotypes that can be corrected by the addition of nickel to free-living cultures. The same bacterium growing on wild-type soybeans or on urease mutants eu1-sun/ eu1-sun or eu4/eu4, each deficient in only one urease isozyme, are urease-positive. These results suggest that the bacterium living on the eu2/eu2 or eu3-e1/eu3-e1 mutant is unable to produce an active urease or hydrogenase because it is effectively starved for nickel. We infer that mutations at Eu2 or Eu3 result in defects in nickel metabolism but not in Ni(2+) uptake or transport, because eu2/eu2 and eu3-e1/eu3-e1 mutants exhibit normal uptake of 63NiCl2. Moreover, wild-type plants grafted on mutant rootstocks produce seeds with fully active urease, indicating unimpeded transport of nickel through mutant roots and stems
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.98.3.942