Chemostat study of kinetics of human lymphokine synthesis in recombinant Escherichia coli

Recombinant technology has made it possible to produce commercially important proteins by fermenting recombinant microorganisms. Before scaling up a fermentation process, it is desirable to find conditions that will optimize recombinant protein production. The chemostat is a device that allows one t...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioengineering 1989-07, Vol.34 (3), p.415-421
Main Authors: Curless, C. E., Forrer, P. D., Mann, M. B., Fenton, D. M., Tsai, L. B.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genetic technics
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
lymphokines
Methods. Procedures. Technologies
Mutant screening
Prokaryotes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recombinant technology has made it possible to produce commercially important proteins by fermenting recombinant microorganisms. Before scaling up a fermentation process, it is desirable to find conditions that will optimize recombinant protein production. The chemostat is a device that allows one to conduct a systematic study of these variables. This article describes a chemostat study of the effect of specific growth rate on the production of a human lymphokine synthesized by a recombinant E. coli .
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260340318