Effects of Growth Hormone on Nuclear Maturation of Ovine Oocytes and Subsequent Embryo Development

• Published in: Reproduction in domestic animals 2010-06, Vol.45 (3), p.530-536
• Main Authors: Shirazi, A, Shams-Esfandabadi, N, Ahmadi, E, Heidari, B
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blastocyst - physiology; Cattle; Cell Nucleus - drug effects; Cell Nucleus - physiology; Culture Media; Drug Interactions; Embryo Culture Techniques - veterinary; Embryonic Development - drug effects; Embryos; Female; Fertilization in Vitro - veterinary; Fetal Blood; Follicle Stimulating Hormone - administration & dosage; Fundamental and applied biological sciences. Psychology; Growth Hormone - administration & dosage; Growth hormones; Mammalian reproduction. General aspects; Oocytes - drug effects; Oocytes - growth & development; Oocytes - ultrastructure; Recombinant Proteins - administration & dosage; Serum; Sheep; Vertebrates: reproduction
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/j.1439-0531.2008.01290.x

The objective of this study was to determine the effect of the presence of recombinant ovine growth hormone either alone or together with follicle stimulating hormone (FSH) during ovine oocyte in vitro maturation (IVM) on nuclear maturation and subsequent embryo development. Moreover, the effect of growth hormine (GH) on embryo development whether influenced by the presence of foetal bovine serum (FBS) was assessed. The abattoir-derived oocytes were randomly divided into four treatment groups and cultured in maturation medium supplemented with: (i) 0.05 IU/ml FSH; (ii) 300 ng/ml roGH; (iii) FSH + roGH; and (iv) no FSH and GH (control). The percentages of germinal vesicle-stage oocytes in GH-treated group after 8 h of culture was significantly higher than the FSH and FSH + GH groups and lower than control (22.4%, 8.7%, 9.1%, and 32% respectively). The percentage of MII-stage oocytes was significantly increased in the presence of GH after 16 and 24 h of culture compared to the control (44.7% and 83.1% vs 32.6% and 73.6% respectively). There was no significant synergism between GH and FSH in terms of nuclear maturation. The blastocyst rates in serum-supplemented groups were enhanced by the presence of FSH and GH compared to the control (35.4% and 31.3 vs 11.4% respectively). Compared with either GH or FSH alone, the subsequent embryo development (blastocyst rate), however, was negatively influenced by co-presence of both hormones (22.8%). In contrast, the corresponding values were not affected in the absence of serum. In conclusion, GH had positive effect on nuclear maturation of sheep oocytes. Moreover, the pattern of the effect of GH on embryo development was influenced by the presence of FBS during IVM.