Multicenter Collaborative Study for Standardization of Candida albicans Genotyping Using a Polymorphic Microsatellite Marker

Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic lad...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2010-07, Vol.48 (7), p.2578-2581
Main Authors: Garcia-Hermoso, Dea, MacCallum, Donna M, Lott, Timothy J, Sampaio, Paula, Serna, Maria-José Buitrago, Grenouillet, Fréderic, Klaassen, Corné H.W, Bretagne, Stéphane
Format: Article
Language:English
Subjects:
Alleles
Bacterial Typing Techniques
Bacterial Typing Techniques - methods
Bacterial Typing Techniques - standards
Biological and medical sciences
Candida albicans
Candida albicans - genetics
Fundamental and applied biological sciences. Psychology
Genotype
Laboratories
Life Sciences
Microbiology
Microbiology and Parasitology
Microsatellite Repeats
Microsatellite Repeats - genetics
Miscellaneous
Molecular Diagnostic Techniques
Molecular Diagnostic Techniques - methods
Molecular Diagnostic Techniques - standards
Mycology
Polymorphism, Genetic
Reference Standards
Reproducibility of Results
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Summary:Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00040-10