Analysis on the Co-Localization of Asbestos Bodies and Fas or CD163 Expression in Asbestos Lung Tissue by in-Air Micro-Pixe

To prevent and control diseases caused by exposure to various agents, it is necessary to determine the harmful level for intervention and to establish a method for measuring that level. In-air microparticle-induced X-ray emission (in-air micro-PIXE) analysis is based on irradiation of specimens with...

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Bibliographic Details
Published in:International journal of immunopathology and pharmacology 2010-01, Vol.23 (1), p.1-11
Main Authors: Matsuzaki, S., Shimizu, Y., Dobashi, K., Nagamine, T., Satoh, T., Ohkubo, T., Yokoyama, A., Ishii, Y., Kamiya, T., Arakawa, K., Makino, S., Utsugi, M., Ishizuka, T., Tanaka, S., Shimizu, K., Mori, M.
Format: Article
Language:English
Subjects:
Aged
Antigens, CD - analysis
Antigens, Differentiation, Myelomonocytic - analysis
Asbestos - analysis
Asbestosis - immunology
Asbestosis - metabolism
Asbestosis - pathology
fas Receptor - analysis
Humans
Immunohistochemistry
Lung - chemistry
Male
Receptors, Cell Surface - analysis
Spectrometry, X-Ray Emission - methods
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Summary:To prevent and control diseases caused by exposure to various agents, it is necessary to determine the harmful level for intervention and to establish a method for measuring that level. In-air microparticle-induced X-ray emission (in-air micro-PIXE) analysis is based on irradiation of specimens with a proton ion microbeam, and has been modified for biological application. Two-dimensional analysis and quantitative analysis using the system confirmed that asbestos induced apoptosis by upregulating Fas expression and also revealed the accumulation of CD163-expressing macrophages in the lungs of patients with asbestosis. By quantitative comparison of the area of Fas or CD163 expression and the Fas- or CD163-negative area in asbestos lung tissue, the harmful levels which caused the expression of Fas or CD163 could be estimated on Silica, Ferrous iron, and Magnesium (the components of asbestos) deposition. These results indicate that the system could be useful for investigating the pathogenesis of inhaled particle-induced immune reactions and for determining harmful levels of exogenous agents.
ISSN:0394-6320
2058-7384
DOI:10.1177/039463201002300101