Suppression of pine wilt disease by an antibacterial agent, oxolinic acid

• Published in: Pest management science 2010-06, Vol.66 (6), p.634-639
• Main Authors: Kwon, Hyeok Ran, Choi, Gyung Ja, Choi, Yong Ho, Jang, Kyoung Soo, Sung, Nack-Do, Kang, Mun Seong, Moon, Yilseong, Lee, Seung Kyu, Kim, Jin-Cheol
• Format: Article
• Language: English
• Subjects: abamectin; Animals; Anti-Bacterial Agents - pharmacology; antibacterial agent; Antibiotics; antimicrobial agents; Bacteria; Bacteria - drug effects; Bacteria - isolation & purification; bacterial diseases of plants; Bacterial Physiological Phenomena; Bacterial plant pathogens; Biological and medical sciences; Bursaphelenchus xylophilus; Control; disease control; forest pests; forest trees; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control; mixed infection; nematicides; Nematoda; Nematodes; oxolinic acid; Oxolinic Acid - pharmacology; Pest control; Phytopathology. Animal pests. Plant and forest protection; pine wilt disease; pine wood nematode; Pinus - microbiology; Pinus densiflora; Plant diseases; Plant Diseases - microbiology; plant parasitic nematodes; plant pathogenic bacteria; Protozoa. Invertebrates; seedlings; strains; tree diseases; tree injection; Trees; Tylenchida - drug effects; Tylenchida - microbiology
• ISSN: 1526-498X; 1526-4998; 1526-4998
• DOI: 10.1002/ps.1920

BACKGROUND: Pine wilt disease (PWD) is very complex and has been reported to be caused by pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, and its accompanying bacteria. However, there is no report on the control of PWD by antibacterial agent. The present study was performed to investigate disease control efficacy of antibacterial agents against PWD.RESULTS: Among six antibacterial antibiotics tested, oxolinic acid (OA) showed the strongest antibacterial activity against five bacteria isolated from three strains of pine wood nematode. In in vivo assay, it effectively suppressed the development of PWD in three-year-old seedlings of Pinus densiflora Sieb. & Zucc.; it showed 71% control when injected at 3 mg per seedling. A mixture of OA and the nematicidal agent abamectin (Ab) showed higher disease control efficacy against PWD than either OA or Ab alone. In addition, OA alone and a mixture of OA and Ab also controlled PWD in approximately 20-year-old pine trees under field conditions.CONCLUSION: This is the first report on the suppression of PWD by OA. The result strongly indicates that PWD could be controlled by antibacterial antibiotic alone and a combination of antibacterial and nematicidal agents. Copyright