Ovulation and pregnancy outcomes in response to human chorionic gonadotropin before resynchronized ovulation in dairy cattle

• Published in: Theriogenology 2010-03, Vol.73 (4), p.449-459
• Main Authors: Buttrey, B.S., Burns, M.G., Stevenson, J.S.
• Format: Article
• Language: English
• Subjects: Animals; artificial insemination; Cattle - blood; Cattle - physiology; Chorionic Gonadotropin - administration & dosage; corpus luteum; Corpus Luteum - physiology; Cow; dairy cows; estrus synchronization; Estrus Synchronization - methods; Female; GnRH; Gonadotropin-Releasing Hormone - administration & dosage; Holstein; Human chorionic gonadotropin; Lactation; Male; Ovarian Follicle - drug effects; Ovarian Follicle - physiology; ovulation; Ovulation Induction - methods; Ovulation Induction - veterinary; Pregnancies per AI; Pregnancy; pregnancy outcome; Progesterone - blood; Random Allocation; Resynchronized ovulation; ultrasonography
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2009.09.027

We first determined a dose of human chorionic gonadotropin (hCG) sufficient to induce ovulation in lactating Holstein cows. Ovaries of 85 previously inseminated cows were mapped using transrectal ultrasonography 7 d before pregnancy diagnosis and assigned randomly to treatments of saline, 100 μg gonadotropin-releasing hormone (GnRH), or 500, 1000, 2000, or 3000 IU hCG. Appearance of new corpus luteum (CL) in response to ≥1000 IU hCG was similar to that for GnRH but greater (P < 0.001) than that for saline. Ovarian structures and serum progesterone then were monitored in 334 previously inseminated Holstein cows 0 and 7 d after treatment with GnRH, hCG (1000 IU), or saline. The incidence of ovulation was greater (P = 0.01) after GnRH than after saline in cows having pretreatment progesterone < 1 ng/mL, whereas in cows having progesterone ≥1 ng/mL, GnRH or hCG was more (P = 0.01) effective than saline, and hCG also differed from GnRH. Holstein cows of unknown pregnancy status in three herds were treated with either GnRH, hCG, or as controls to initiate an ovulation-resynchronization procedure 7 d before pregnancy diagnosis. In 1109 treated pregnant cows, pregnancy loss during 4 wk after treatment tended (P = 0.06) to be greater in those treated with hCG. Treated cows (n = 1343) diagnosed not pregnant were then given prostaglandin F 2α and inseminated and received GnRH 72 h later. A treatment by herd interaction (P = 0.06) resulted in more pregnancies after GnRH in two herds and after hCG in one herd compared with saline. We concluded that (1) ≥ 1000 IU hCG resulted in more CL than did treatment with saline, and the incidence of new CL after either GnRH or hCG depended on pretreatment progesterone status; (2) hCG tended to increase pregnancy loss in pregnant cows; and (3) pregnancies per artificial insemination after initiating resynchronization with either hCG or GnRH produced ambiguous results.