ICP-MS-Based strategies for protein quantification

In the post-genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on iso...

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Bibliographic Details
Published in:Mass spectrometry reviews 2010-03, Vol.29 (2), p.326-348
Main Authors: Wang, Meng, Feng, Wei-Yue, Zhao, Yu-Liang, Chai, Zhi-Fang
Format: Article
Language:English
Subjects:
Algorithms
Analytical, structural and metabolic biochemistry
Biological and medical sciences
elemental labeling
elemental mass spectrometry
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
isotope dilution
laser ablation
molecular mass spectrometry
Peptide Mapping - methods
Peptide Mapping - trends
Proteins
Proteins - analysis
Proteins - chemistry
quantitative proteomics
Spectrometry, Mass, Electrospray Ionization - instrumentation
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:In the post-genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on isotope labeling combined with molecular mass spectrometry. Recently, a remarkable progress that utilizes inductively coupled plasma-mass spectrometry (ICP-MS) as an attractive complement to electrospray MS and MALDI MS for protein quantification, especially for absolute quantification, has been achieved. This review will selectively discuss the recent advances of ICP-MS-based technique, which will be expected to further mature and to become one of the key methods in quantitative proteomics.
ISSN:0277-7037
1098-2787
DOI:10.1002/mas.20241