Incorporation of Bovine Bone Marrow Stromal Cells into Porcine Foetal Tissues after Xenotransplantation

Summary Bovine bone marrow stromal cells (BMSCs) were injected into the liver of foetal pigs at about 40 days of gestation to test whether these cells could populate developing tissue, and if so, which ones. Approximately 40 days after injection, the foetuses were harvested and tissue sections from...

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Bibliographic Details
Published in:Anatomia, histologia, embryologia histologia, embryologia, 2003-04, Vol.32 (2), p.98-101
Main Authors: Troyer, D. L., Weiss, M. L., Mitchell, K. E., Martin, P. L., Davis, D. L.
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Transplantation
Cattle - genetics
DNA - analysis
DNA Primers
Embryonic and Fetal Development
Fetus
Male
Polymerase Chain Reaction - veterinary
Stromal Cells - transplantation
Swine - embryology
Swine - genetics
Transplantation, Heterologous - methods
Transplantation, Heterologous - veterinary
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Summary:Summary Bovine bone marrow stromal cells (BMSCs) were injected into the liver of foetal pigs at about 40 days of gestation to test whether these cells could populate developing tissue, and if so, which ones. Approximately 40 days after injection, the foetuses were harvested and tissue sections from many areas of the body were analysed for the presence of bovine cells using two different methods. First, using PCR, bovine repetitive DNA was found to be present in DNA extracted from foetal pig tissues. Secondly, using oligonucleotide primed in situ synthesis (PRINS), the in situ presence of bovine cells was found within porcine tissue sections. PRINS‐labelled cells were found within cartilage, perichondrium, connective tissue and smooth muscle. These data suggest that bovine BMSCs integrate throughout the foetal pig.
ISSN:0340-2096
1439-0264
DOI:10.1046/j.1439-0264.2003.00428.x