A highly branched 1,3-β- d-glucan extracted from Aureobasidium pullulans induces cytokine production in DBA/2 mouse-derived splenocytes

• Published in: International immunopharmacology 2009-11, Vol.9 (12), p.1431-1436
• Main Authors: Tada, Rui, Yoshikawa, Masashi, Kuge, Takao, Tanioka, Asuka, Ishibashi, Ken-ichi, Adachi, Yoshiyuki, Tsubaki, Kazufumi, Ohno, Naohito
• Format: Article
• Language: English
• Subjects: Animals; Aureobasidium pullulans; beta-Glucans - administration & dosage; Biological and medical sciences; Cells, Cultured; Cytokine production; Cytokines - secretion; DBA/2 mice; Fungi - chemistry; Immunization; Infection - immunology; Infection - therapy; Male; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Neoplasms - immunology; Neoplasms - therapy; Pharmacology. Drug treatments; Primary structure of β-glucans; Spleen - drug effects; Spleen - immunology; Spleen - metabolism; Spleen - pathology; Th1 Cells - drug effects; Th1 Cells - immunology; Th1 Cells - metabolism; Th1 Cells - pathology; β- d-Glucan
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2009.08.014

We recently elucidated the structure of a highly branched 1,3-β- d-glucan with 6-monoglucopyranosyl side chains, extracted from Aureobasidium pullulans (AP-FBG). Although the biological effects of β- d-glucans are known to depend on their structures, the effects of a highly branched 1,3-β- d-glucan on the production of cytokines by leukocytes in mice have not yet been elucidated. In this study, we found that AP-FBG strongly induced the production of various cytokines, especially Th1 cytokines (e.g., IFN-γ and IL-12p70) and Th17 cytokines (e.g., IL-17A), but did not induce the production of IL-4, IL-10, and TNF-α in DBA/2 mouse-derived splenocytes in vitro.