Continuous cell suspension processing using magnetically stabilized fluidized beds

The behavior of a cell suspension in a continuous magnetically stabilized fluidized bed (MSFB) was investigated both experimentally and theoretically. The low, constant pressure drop and fluidity of the solids phase in the MSFB allowed a continuous countercurrent separator to be constructed. The mag...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1991-01, Vol.37 (2), p.110-120
Main Authors: Terranova, Brenda E., Burns, Mark A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cell suspensions
continuous culture
fluidized beds
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
process engineering
Saccharomyces cerevisiae
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Summary:The behavior of a cell suspension in a continuous magnetically stabilized fluidized bed (MSFB) was investigated both experimentally and theoretically. The low, constant pressure drop and fluidity of the solids phase in the MSFB allowed a continuous countercurrent separator to be constructed. The magnetic field eliminated all motion of the solids phase (nickel spheres) and produced a device similar to a packed‐bed depth filter. Yeast cells were used as the suspended solids and the performance of the MSFB filter was assessed as a function of the bed height, solids velocity, cell concentration, and liquid composition. Removal rates could be adjusted by controlling the cell/support interaction and were found to be as high as 99%. A mathematical model was used to aid in understanding this filtration and was found to agree qualitatively with all experimental observations. Comparison of the model with the data suggests that both cell/cell binding and cell shadowing are occurring.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260370204