Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells

The routine measurement of the cell size distribution of a Chinese hamster ovary (CHO) cell population during a repeated batch process enables the predetermination of exponential growth even 24 h before the population enters the log phase, due to a short but significantly increased cell size during...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1997-09, Vol.55 (5), p.793-797
Main Authors: Seewöster, Thomas, Lehmann, Jürgen
Format: Article
Language:English
Subjects:
Ammonia
Animal cells
Biological and medical sciences
Biotechnology
cell size
cell synchronization
CHO
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
repeated batch
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Summary:The routine measurement of the cell size distribution of a Chinese hamster ovary (CHO) cell population during a repeated batch process enables the predetermination of exponential growth even 24 h before the population enters the log phase, due to a short but significantly increased cell size during the lag phase. A prolongation of the stationary phase causes to progressive limitation in asparagine, serine, and ethanolamine. Such extended limitation influences the duration of the following lag phase and obviously induces a synchronization of the cell population that can be monitored easily by a fast cell size analyzing technique. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 793–797, 1997.
ISSN:0006-3592
1097-0290
DOI:10.1002/(SICI)1097-0290(19970905)55:5<793::AID-BIT9>3.0.CO;2-6