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Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells

The routine measurement of the cell size distribution of a Chinese hamster ovary (CHO) cell population during a repeated batch process enables the predetermination of exponential growth even 24 h before the population enters the log phase, due to a short but significantly increased cell size during...

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Published in:	Biotechnology and bioengineering 1997-09, Vol.55 (5), p.793-797
Main Authors:	Seewöster, Thomas, Lehmann, Jürgen
Format:	Article
Language:	English
Subjects:	Ammonia Animal cells Biological and medical sciences Biotechnology cell size cell synchronization CHO Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous repeated batch
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creator	Seewöster, Thomas Lehmann, Jürgen
description	The routine measurement of the cell size distribution of a Chinese hamster ovary (CHO) cell population during a repeated batch process enables the predetermination of exponential growth even 24 h before the population enters the log phase, due to a short but significantly increased cell size during the lag phase. A prolongation of the stationary phase causes to progressive limitation in asparagine, serine, and ethanolamine. Such extended limitation influences the duration of the following lag phase and obviously induces a synchronization of the cell population that can be monitored easily by a fast cell size analyzing technique. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 793–797, 1997.
doi_str_mv	10.1002/(SICI)1097-0290(19970905)55:5<793::AID-BIT9>3.0.CO;2-6
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subjects	Ammonia Animal cells Biological and medical sciences Biotechnology cell size cell synchronization CHO Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous repeated batch
title	Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells
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