Characterisation of the expression of a novel constitutive maize promoter in transgenic wheat and maize

A novel constitutive promoter from the maize histone H2Bgene was recently identified. In this study, we characterised H2B promoter activity in both wheat and maize tissues using the gusA reporter gene and two synthetic versions of the pat (phosphinothricin acetyl transferase) selectable marker gene,...

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Bibliographic Details
Published in:Plant cell reports 2003-02, Vol.21 (6), p.569-576
Main Authors: RASCO-GAUNT, S, LIU, D, KREBBERS, E, LAZZERI, P, JAYNE, S, RICE, D, LI, C. P, DOHERTY, A, HAGEMANN, K, RILEY, A, THOMPSON, T, BRUNKAN, C, MITCHELL, M, LOWE, K
Format: Article
Language:English
Subjects:
Acetyltransferases - genetics
Acetyltransferases - metabolism
Biological and medical sciences
Corn
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Genetic Markers - genetics
Glucuronidase - genetics
Glucuronidase - metabolism
Histones - genetics
Histones - metabolism
Introns - genetics
Plants, Genetically Modified - genetics
Promoter Regions, Genetic - genetics
Transformation, Genetic
Transgenic plants
Triticum - genetics
Wheat
Zea mays - genetics
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Summary:A novel constitutive promoter from the maize histone H2Bgene was recently identified. In this study, we characterised H2B promoter activity in both wheat and maize tissues using the gusA reporter gene and two synthetic versions of the pat (phosphinothricin acetyl transferase) selectable marker gene, namely mopat and popat. Analyses of transgenic plants showed that the H2B promoter is able to drive the expression of gusA to strong, constitutive levels in wheat and maize tissues. Using an H2B:mopat construct and phosphinothricin selection, we recovered transgenic wheat plants at efficiencies ranging from 0.3% to 7.4% (mean 1.6%), and the efficiency of selection ranged from 40% to 100% (mean 77.7%). In another application, H2B was combined with the maize Ubi-1 or the maize Adh-1 intron to drive the expression of mopat and popat. Transformation efficiencies with the Ubi-1 intron were between 1.4- to 16-fold greater than with the Adh-1 intron. However, the use of either of the introns was necessary for the recovery of transgenic plants. Mopat gave higher transformation efficiencies and induced higher levels of PAT protein in maize tissues than popat.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-002-0552-y