Proteasomal activity in skeletal muscle: A matter of assay design, muscle type, and age

The ubiquitin–proteasome system (UPS) is a major degradation system for regulatory and misfolded proteins. UPS function has been implicated to exert a central role in the pathogenesis of various human diseases. Because biochemical analyses are often hampered by the amount of available diseased tissu...

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Bibliographic Details
Published in:Analytical biochemistry 2010-04, Vol.399 (2), p.225-229
Main Authors: Strucksberg, Karl-Heinz, Tangavelou, Karthikeyan, Schröder, Rolf, Clemen, Christoph S.
Format: Article
Language:English
Subjects:
Age Factors
Animals
Luminescent Measurements - methods
Male
Mice
Muscle, Skeletal - enzymology
Proteasomal activity
Proteasome Endopeptidase Complex - chemistry
Proteasome Endopeptidase Complex - metabolism
Proteasome inhibitor
Protein degradation
Skeletal muscle
Ubiquitin proteasome system
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Summary:The ubiquitin–proteasome system (UPS) is a major degradation system for regulatory and misfolded proteins. UPS function has been implicated to exert a central role in the pathogenesis of various human diseases. Because biochemical analyses are often hampered by the amount of available diseased tissue, we report on the establishment and validation of a luminescence-based proteasomal activity assay applicable to 5-mg quantities of skeletal muscle. We demonstrate that the specific proteasomal activity differs in individual muscle groups and decreases with aging. These findings warrant the use of appropriate controls and a careful interpretation of results in mammalian skeletal muscle pathologies.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2009.12.026