Exogenous polyamines elicit herbivore-induced volatiles in lima bean [Phaseolus lunatus] leaves: Involvement of calcium, H2O2 and jasmonic acid

• Published in: Plant and cell physiology 2009-12, Vol.50 (12), p.2183-2199
• Main Authors: Ozawa, R., Kyoto Univ., Otsu (Japan). Center for Ecological Research, Bertea, C.M, Foti, M, Narayana, R, Arimura, G, Muroi, A, Horiuchi, J, Nishioka, T, Maffei, M.E, Takabayashi, J
• Format: Article
• Language: English
• Subjects: ACIDE JASMONIQUE; ACIDO JASMONICO; Animals; CALCIO; CALCIUM; Calcium - metabolism; Calcium and ROS signaling • Herbivore-induced volatile organic compounds • Jasmonic acid • Lima bean • Polyamines • Quantitative gene expression; COMPOSE VOLATIL; COMPUESTO VOLATIL; Cyclopentanes - pharmacology; DEPREDADORES; Gene Expression Regulation, Plant; HERBIVORE; HERBIVORES; HERBIVOROS; http://www.fao.org/aos/agrovoc#c_1196; http://www.fao.org/aos/agrovoc#c_24933; http://www.fao.org/aos/agrovoc#c_26721; http://www.fao.org/aos/agrovoc#c_30903; http://www.fao.org/aos/agrovoc#c_32517; http://www.fao.org/aos/agrovoc#c_3567; http://www.fao.org/aos/agrovoc#c_36637; http://www.fao.org/aos/agrovoc#c_5766; http://www.fao.org/aos/agrovoc#c_6163; Hydrogen Peroxide - metabolism; JASMONIC ACID; Oxylipins - pharmacology; Phaseolus - drug effects; Phaseolus - enzymology; Phaseolus - genetics; Phaseolus - metabolism; PHASEOLUS LUNATUS; PHYTOSEIULUS PERSIMILIS; POLIAMINAS; POLYAMINE; POLYAMINES; Polyamines - pharmacology; PREDATEUR; PREDATORS; Reactive Oxygen Species - metabolism; Terpenes - metabolism; Tetranychidae; TETRANYCHUS URTICAE; VOLATILE COMPOUNDS; Volatilization
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcp153

We investigated the role of polyamines (PAs) in lima bean (Phaseolus lunatus) leaves on the production of herbivorous mite (Tetranychus urticae)-induced plant volatiles that attract carnivorous natural enemies of the herbivores. To do this, we focused on the effects of the exogenous PAs [cadaverine, putrescine, spermidine and spermine (Spm)] on the production of volatiles, H2O2 and jasmonic acid (JA) and the levels of defensive genes, cytosolic calcium and reactive oxygen species (ROS). Among the tested PAs, Spm was the most active in inducing the production of volatile terpenoids known to be induced by T. urticae. An increase in JA levels was also found after Spm treatment, indicating that Spm induces the biosynthesis of JA, which has been shown elsewhere to regulate the production of some volatile terpenoids. Further, treatment with JA and Spm together resulted in greater volatile emission than that with JA alone. In a Y-tube olfactometer, leaves treated with Spm + JA attracted more predatory mites (Phytoseiulus persimilis) than those treated with JA alone. After treatment with Spm + JA, no effects were found on the enzyme activity of polyamine oxidase and copper amine oxidase. However, induction of calcium influx and ROS production, and increased enzyme activities and gene expression for NADPH oxidase complex, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase were found after treatment with Spm + JA. These results indicate that Spm plays an important role in the production of T. urticae-induced lima bean leaf volatiles.