Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure to Bacillus thuringiensis that relates in general to crystal protein gene content

Seventy strains of Bacillus thuringiensis representing 21 serovars were allocated to 38 genomic groups using pulsed field gel electrophoresis (PFGE) of restriction enzyme-digested DNA. There was a broad correlation between PFGE type and serotype for serovars darmstadiensis, israelensis, kenyae, kuma...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-06, Vol.223 (1), p.61-66
Main Authors: Gaviria Rivera, Adelaida M, Priest, Fergus G
Format: Article
Language:English
Subjects:
Action of physical and chemical agents
Bacillus thuringiensis
Bacillus thuringiensis - classification
Bacillus thuringiensis - genetics
Bacterial Proteins - genetics
Bacterial Toxins
Biological and medical sciences
Chromosomes, Bacterial
clonal group
clones
complement
crystal protein
crystal proteins
DNA
DNA Fingerprinting
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Endotoxins - genetics
Fundamental and applied biological sciences. Psychology
genes
Genome, Bacterial
Hemolysin Proteins
insect pathogenicity
Microbiology
Mycological methods and techniques used in mycology
Mycology
polymerase chain reaction
population structure
pulsed-field gel electrophoresis
serotypes
Serotyping
Virology
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Summary:Seventy strains of Bacillus thuringiensis representing 21 serovars were allocated to 38 genomic groups using pulsed field gel electrophoresis (PFGE) of restriction enzyme-digested DNA. There was a broad correlation between PFGE type and serotype for serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, sotto, thuringiensis, and tolworthi, although some serovars included atypical strains. Serovars canadensis and entomocidus were heterogeneous. Detection of crystal protein genes by polymerase chain reaction indicated an approximate correlation between PFGE type and cry gene complement. For example, cry1 products were amplified from DNA from PFGE type 17 strains of serovar aizawai and from PFGE type 23 strains of serovar tolworthi but not from a PFGE 18 strain of aizawai nor from a PFGE type 24 strain of tolworthi. These data suggest a clonal population structure to B. thuringiensis with some consistency of Cry-plasmid composition within PFGE types.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00347-1