Sotrastaurin and cyclosporine drug interaction study in healthy subjects

• Published in: Biopharmaceutics & drug disposition 2010-07, Vol.31 (5-6), p.331-339
• Main Authors: Kovarik, John M., Stitah, Sylvie, Slade, Alan, Vitaliti, Alessandra, Straube, Frank, Grenet, Olivier, Winter, Serge, Sfikas, Nikolaos, Seiberling, Michael
• Format: Article
• Language: English
• Subjects: Adult; Biomarkers, Pharmacological - blood; cyclosporine; Cyclosporine - administration & dosage; Cyclosporine - blood; Cyclosporine - pharmacokinetics; Cyclosporine - pharmacology; Dose-Response Relationship, Drug; Drug Combinations; Drug Interactions; Humans; Lymphocyte Activation - drug effects; Male; protein kinase C; Pyrroles - administration & dosage; Pyrroles - blood; Pyrroles - pharmacokinetics; Pyrroles - pharmacology; Quinazolines - administration & dosage; Quinazolines - blood; Quinazolines - pharmacokinetics; Quinazolines - pharmacology; sotrastaurin; T-Lymphocytes - drug effects; T-Lymphocytes - metabolism
• ISSN: 0142-2782; 1099-081X
• DOI: 10.1002/bdd.715

Introduction. Sotrastaurin is an immunosuppressant that inhibits protein kinase C and blocks T‐lymphocyte activation. The authors determined the effect of combining sotrastaurin with the calcineurin inhibitor cyclosporine on the pharmacokinetics and biomarker responses to both drugs. Methods. This was a randomized, 4‐period, crossover study in 20 healthy subjects who received single oral doses of (1) sotrastaurin 100 mg, (2) cyclosporine 400 mg, (3) 100 mg sotrastaurin with 100 mg cyclosporine and (4) 100 mg sotrastaurin with 400 mg cyclosporine. Blood samples were collected to measure drug levels and biomarkers of T‐lymphocyte activation (interleukin‐2 and tumor necrosis factor producing T‐cells and interleukin‐2 messenger RNA levels) and of T‐lymphocyte proliferation (thymidine uptake). Results. Sotrastaurin did not alter cyclosporine AUC; however, low‐dose and high‐dose cyclosporine increased sotrastaurin AUC by 1.2‐fold [90% confidence interval, 1.1–1.4] and 1.8‐fold [1.6–2.1], respectively. Adding high‐dose cyclosporine to a low‐therapeutic dose of sotrastaurin significantly enhanced the inhibition of cytokine production by 31% [95% confidence interval, 25–36%], of interleukin‐2 messenger RNA levels by 13% [7–19%], and of thymidine uptake by 37% [32–42%] compared with sotrastaurin alone. Addition of low‐dose cyclosporine elicited slightly lower enhancements in inhibition by 21% [14–28%], 6% [−4–16%], and 26% [21–30%], respectively, compared with sotrastaurin alone. Conclusions. Sotrastaurin did not alter the pharmacokinetics of cyclosporine, but cyclosporine increased sotrastaurin AUC up to 1.8‐fold. The combined drugs elicited a significantly greater inhibition of T‐cell activation and proliferation than sotrastaurin alone. Copyright © 2010 John Wiley & Sons, Ltd.