Probiotic properties of Lactobacillus spp isolated from gastric biopsies of Helicobacter pylori infected and non-infected individuals

Gastric microbiota is a complex ecosystem, composed by diverse microorganisms, where H pylori and Lactobacillus are relevant species. To study the probiotic properties of Lactobacillus spp isolated from gastric biopsies of subjects bearing or not H pylori. Gastric biopsies from 197 individuals that...

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Bibliographic Details
Published in:Revista medíca de Chile 2009-03, Vol.137 (3), p.369-376
Main Authors: García C, Apolinaria, Henríquez A, Patricio, Retamal R, Carolina, Pineda C, Susana, Delgado Sen, Carolina, González C, Carlos
Format: Article
Language:Spanish
Subjects:
Adolescent
Bacterial Physiological Phenomena
Biopsy
Colony Count, Microbial
Gastric Mucosa - microbiology
Helicobacter Infections - microbiology
Helicobacter Infections - pathology
Helicobacter pylori
Helicobacter pylori - growth & development
Helicobacter pylori - isolation & purification
Humans
Lactobacillus
Lactobacillus - isolation & purification
Lactobacillus - metabolism
Lactobacillus - physiology
Probiotics - metabolism
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Summary:Gastric microbiota is a complex ecosystem, composed by diverse microorganisms, where H pylori and Lactobacillus are relevant species. To study the probiotic properties of Lactobacillus spp isolated from gastric biopsies of subjects bearing or not H pylori. Gastric biopsies from 197 individuals that consulted for gastrointestinal disorders were cultivated in media specific for H pylori and Lactobacillus spp. Probiotic properties of lactobacilli isolates and histological diagnosis of H pylori colonized patients were performed. Among 197 individuals evaluated, Hpylori was detected in 43%, Lactobacillus spp in 24%, while 8% presented both bacteria simultaneously. Forty one percent of the Lactobacillus spp strains produced hydrogen peroxide, S'3% presented a highly hydrophobic surface and 32% had an inhibitory effect upon Hpylori ATCC 43504, even greater than Lactobacillus LGG or LAL reference strains. The main pathological diagnosis of patients ivas chronic non-atrophic gastritis (73%) followed by intestinal metaplasia (19%). A similar proportion of both conditions were observed in patients colonized by Lactobacillus spp, while not colonized individuals developed preferentially chronic non-atrophic gastritis (p =0.002). Lactobacillus spp and H pylori were mutually excluded, suggesting a competitive colonization model between probiotic bacteria and pathogens .
ISSN:0034-9887