Effects of breed, sex and halothane genotype on fatty acid composition of triacylglycerols and phospholipids in pork longissimus muscle

• Published in: Journal of animal breeding and genetics (1986) 2009-08, Vol.126 (4), p.259-268
• Main Authors: Zhang, S, Knight, T.J, Stalder, K.J, Goodwin, R.N, Lonergan, S.M, Beitz, D.C
• Format: Article
• Language: English
• Subjects: Animal Feed; Animals; Biochemistry; Breed; Breeding of animals; fatty acid; Fatty acids; Fatty Acids - analysis; Female; Gender; Genotype; Genotype & phenotype; Halothane; Hogs; longissimus muscle; Male; Muscles - chemistry; Muscular system; phospholipids; Phospholipids - chemistry; pig; Sex Characteristics; swine; Swine - anatomy & histology; Swine - classification; Swine - genetics; triacylglycerols; Triglycerides - chemistry
• ISSN: 0931-2668; 1439-0388
• DOI: 10.1111/j.1439-0388.2008.00782.x

The objective of this study was to estimate the effects of breed, sex, and halothane (HAL-1843TM) genotype on fatty acid composition of triacylglycerols (TAG) and phospholipids (PL) extracted from porcine longissimus muscle (LM). Purebred Yorkshire (n = 131), Duroc (n = 136), Hampshire (n = 49), Spotted (n = 35), Chester White (n = 74), Poland China (n = 51), Berkshire (n = 169) and Landrace (n = 82) pigs (n = 727; 427 barrows and 300 gilts) from the 1994 and 2001 National Barrow Show Sire Progeny Tests were used. For statistical analyses, a mixed model was used that included fixed effects of breed, sex, HAL-1843TM genotype, year, slaughter date within each year, interaction of breed x sex and random effects of sire and dam within breed. Breeds and sex were significantly associated with the percentages of the majority fatty acids in TAG. Duroc pigs had greater total saturated fatty acids (SFA) and lower total monounsaturated fatty acids (MUFA) (p < 0.05) contents than did pigs of all other breeds except Berkshire (p > 0.05). The concentration of total polyunsaturated fatty acids (PUFA) was the greatest in Hampshire pigs (p < 0.05). The content of total SFA was greater (p < 0.01), whereas the concentrations of total MUFA and PUFA were lower (p < 0.01) in barrows than those in gilts. The contents of major SFA in PL did not differ significantly among pigs from different breeds and sex groups. However, breed and sex significantly affected the concentrations of major MUFA and PUFA in PL and strong negative correlation between the total contents of MUFA and PUFA in PL was observed in the current study. Chester White pigs had greater total MUFA and lower total PUFA contents (p < 0.05) in PL than did pigs of all other breeds except Spotted (p > 0.05). In contrast to breed and sex effects, the concentrations of fatty acids in PL were more affected by HAL-1843TM genotype than those in TAG. The content of C16:0, a major SFA in PL, differed significantly in pigs with different HAL-1843TM genotypes. In conclusion, these results suggest that breed and sex are important sources of the variations for fatty acid composition of TAG and PL in LM.