Evaluation of Trypsin Treatment on the Inactivation of Bovine Herpesvirus Type 1 on In Vitro Produced Pre-implantation Embryos

Contents The aim of this study was to evaluate the efficiency of trypsin treatment on the inactivation of bovine herpesvirus type 1 (BoHV‐1) on in vitro produced by fertilization and artificially infected bovine embryos. Bovine embryos on day 7 were exposed with 10 μl of BoHV‐1, Los Angeles strain 1...

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Bibliographic Details
Published in:Reproduction in domestic animals 2009-06, Vol.44 (3), p.536-539
Main Authors: D'Angelo, M, Visintin, JA, Richtzenhain, LJ, Gonçalves, RF
Format: Article
Language:English
Subjects:
Animal diseases
Animal reproduction
Animals
Antiviral Agents - pharmacology
Biological and medical sciences
Blastocyst - virology
Cattle
Cattle - embryology
Cattle - virology
Cell Line
DNA, Viral - analysis
Drug therapy
Embryos
Fertilization in Vitro - veterinary
Fundamental and applied biological sciences. Psychology
Herpes viruses
Herpesvirus 1, Bovine - drug effects
Herpesvirus 1, Bovine - genetics
Herpesvirus 1, Bovine - isolation & purification
Kidney
Mammalian reproduction. General aspects
Polymerase Chain Reaction
Reproductive technologies
Trypsin - pharmacology
Vertebrates: reproduction
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Summary:Contents The aim of this study was to evaluate the efficiency of trypsin treatment on the inactivation of bovine herpesvirus type 1 (BoHV‐1) on in vitro produced by fertilization and artificially infected bovine embryos. Bovine embryos on day 7 were exposed with 10 μl of BoHV‐1, Los Angeles strain 107.5 TCID. These embryos and control embryos were divided in two groups: submitted to the sequential washes or to the trypsin treatment according to the International Embryo Transfer Society (IETS) guidelines. The embryos and the last washing drop of each group were used as inoculum to infect Madin Darby bovine kidney (MDBK) cells and submitted to nested PCR reaction using the primer that encodes the gene conserved region of virus glycoprotein gB. The data have shown that the control embryos and their last washing drop were negative. The exposed embryos that were treated with trypsin have shown positive results on the n‐PCR and MDBK culture, and their last washing drop were negative. Our data have demonstrated that the trypsin treatment was not able to eliminate the BHV‐1 of the embryos, suggesting an interaction between virus and embryo.
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2008.01110.x