Taste receptor cell-based biosensor for taste specific recognition based on temporal firing

Taste receptor cells are the taste sensation elements expressing sour, salty, sweet, bitter and umami receptors, respectively. There are cell-to-cell communications between different types of cells. Nevertheless, the mechanism of taste sensation and taste information coded by taste receptor cell is...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2009-09, Vol.25 (1), p.228-233
Main Authors: Chen, Peihua, Wang, Bingqing, Cheng, Gong, Wang, Ping
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Biological and medical sciences
Biosensing Techniques - methods
Biosensor
Biosensors
Biotechnology
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Rats
Rats, Sprague-Dawley
Taste
Taste Buds - cytology
Taste Buds - metabolism
Taste receptor cell-based biosensor
Taste sensation
Temporal firing
Various methods and equipments
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Summary:Taste receptor cells are the taste sensation elements expressing sour, salty, sweet, bitter and umami receptors, respectively. There are cell-to-cell communications between different types of cells. Nevertheless, the mechanism of taste sensation and taste information coded by taste receptor cell is not well understood at present and it is a long-standing issue. In order to explore taste sensation and analyze taste-firing responses from another point of view, we present a promising biomimetic taste receptor cell-based biosensor. The temporal firing responses to different tastants are recorded. Meanwhile, we investigate the firing rate and temporal firing of taste receptor cells. The experimental results are consistent with that from patch clamp and molecular biology experiment. Firing rate is dependent on the concentration of stimulus. PCA analysis (principal component analysis) of the temporal firing responses shows that the responses from different types of taste receptor cells can be distinguished. Furthermore, exogenous ATP is applied to mimic the effects of transmitter ATP (adenosine triphosphate) released from type II cells onto type III cells. Both enhanced and inhibitory effects on spontaneous firing are observed. This novel biomimetic hybrid biosensor provides a potential solution to investigate the taste sensation and coding mechanisms in a non-invasive way.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2009.05.034