Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

A simple and specific LC‐DAD‐ESI‐MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the...

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Bibliographic Details
Published in:Biomedical chromatography 2010-05, Vol.24 (5), p.479-489
Main Authors: Hu, Yong Mei, Wang, Yi Tao, Sze, Stephen C. W., Tsang, K. W., Wong, H. K., Liu, Qing, Zhong, Li Dan, Tong, Yao
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Chromatography, Liquid - methods
Drugs, Chinese Herbal - administration & dosage
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - metabolism
Drugs, Chinese Herbal - pharmacokinetics
Erxian Decoction (EXD)
Female
LC-DAD-ESI-MS
menopausal syndromes
metabolites
pharmacokinetic study
Rats
Rats, Sprague-Dawley
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
traditional Chinese medicine prescriptions (TCMP)
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Summary:A simple and specific LC‐DAD‐ESI‐MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI‐MSn analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MSn fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the effective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.1315