Harmonization of values for serum alkaline phosphatase catalytic activity concentration employing commutable calibration materials

Harmonization of results allows a more effective utilization of laboratory tests; we verified the feasibility of harmonizing serum alkaline phosphatase results by two methods. Patient sera (n=106) and candidate calibration materials (n=8) were analyzed by two methods, employing either diethanolamine...

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Bibliographic Details
Published in:Clinica chimica acta 2010-06, Vol.411 (11-12), p.882-885
Main Authors: Cattozzo, Giampaolo, Albeni, Chiara, Franzini, Carlo
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Alkaline Phosphatase - blood
Alkaline Phosphatase - standards
Calibration
Calibration materials
Catalysis
Commutability
Enzyme Activation - physiology
Enzymes
Ethanolamines - analysis
Ethanolamines - standards
Harmonization of test results
Humans
Propanolamines - analysis
Propanolamines - standards
Technology, Pharmaceutical - methods
Technology, Pharmaceutical - standards
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Summary:Harmonization of results allows a more effective utilization of laboratory tests; we verified the feasibility of harmonizing serum alkaline phosphatase results by two methods. Patient sera (n=106) and candidate calibration materials (n=8) were analyzed by two methods, employing either diethanolamine (DEA) or 2-amino-2-methyl-1-propanol (AMP) as phosphate-accepting buffers. Results for patient sera by the DEA method were recalculated, with either a commutable or a non-commutable calibration material, both with values assigned by the AMP method. After calibration with the commutable material, the median intermethod difference (DEA–AMP) and ratio (DEA/AMP) dropped from 195U/l to 0U/l and from 2.47 to 1.00, respectively. When a non-commutable material was used the former became 124U/l and the latter 1.94. After recalibration with the commutable material, linear regression and correlation analysis of DEA vs AMP values for the set of 106 patient sera gave: intercept=0.8U/l; slope=0.997; and nonparametric correlation coefficient r=0.9995. Harmonization of alkaline phosphatase results by AMP and DEA methods is feasible when commutable calibration materials are used in the trueness transfer process.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2010.03.008