Definition of a Tumor Suppressor Locus Within Human Chromosome 3p21-p22

Cytogenetic abnormalities and high-frequency allele losses involving the short arm of human chromosome 3 have been identified in a variety of histologically different neoplasms. These findings suggest that a tumor-suppressor gene or genes may be located in the region of 3p14-p25, although there has...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-11, Vol.89 (22), p.10877-10881
Main Authors: Killary, Ann McNeill, Wolf, M. Elizabeth, Giambernardi, Troy A., Naylor, Susan L.
Format: Article
Language:English
Subjects:
Adenine Phosphoribosyltransferase - genetics
Alleles
Animals
Base Sequence
Biological and medical sciences
Cell Line
Cell lines
Chromatin. Chromosome
chromosome 3
Chromosome Banding
Chromosome Mapping
Chromosomes
Chromosomes, Human, Pair 3
Clone Cells
fibroblasts
Fundamental and applied biological sciences. Psychology
Genes
Genes, Tumor Suppressor
Genetic loci
Genetics
Human chromosomes
Humans
Hybrid Cells
Hybridity
Hypoxanthine Phosphoribosyltransferase - genetics
Karyotyping
localization
man
Mice
Mice, Nude
Microcell hybrids
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Neoplasms, Experimental - genetics
Neoplasms, Experimental - pathology
Oligodeoxyribonucleotides
Polymerase Chain Reaction
tumor suppressor genes
Tumors
Citations: Items that cite this one
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Description
Summary:Cytogenetic abnormalities and high-frequency allele losses involving the short arm of human chromosome 3 have been identified in a variety of histologically different neoplasms. These findings suggest that a tumor-suppressor gene or genes may be located in the region of 3p14-p25, although there has been no definitive functional proof for the involvement of a particular region of 3p. We report a rapid genetic assay system that has allowed functional analysis of defined regions of 3p in the suppression of tumorigenicity in vivo. Interspecific microcell hybrids containing fragments of chromosome 3p were constructed and screened for tumorigenicity in athymic nude mice. Hybrid clones were obtained that showed a dramatic tumor suppression and contained a 2-megabase fragment of human chromosomal material encompassing the region 3p21 near the interface with 3p22. With these hybrid clones, we have defined a genetic locus at 3p21-p22 intimately involved in tumor suppression.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.22.10877