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Ionizing radiation enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulations of death receptor 4 (DR4) and death receptor 5 (DR5) in human osteosarcoma cells
Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor‐related apoptosis‐inducing ligand (...
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| Published in: | Journal of orthopaedic research 2010-06, Vol.28 (6), p.739-745 |
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| Main Authors: | , , , , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c4636-a602e07b6968c7821ca0944e5e193c8fa45539976540c2f4a021eba2ad61ae173 |
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| cites | cdi_FETCH-LOGICAL-c4636-a602e07b6968c7821ca0944e5e193c8fa45539976540c2f4a021eba2ad61ae173 |
| container_end_page | 745 |
| container_issue | 6 |
| container_start_page | 739 |
| container_title | Journal of orthopaedic research |
| container_volume | 28 |
| creator | Hori, Takeshi Kondo, Takashi Kanamori, Masahiko Tabuchi, Yoshiaki Ogawa, Ryohei Zhao, Qing-Li Ahmed, Kanwal Yasuda, Taketoshi Seki, Shoji Suzuki, Kayo Kimura, Tomoatsu |
| description | Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL‐induced Bid and caspase‐3 activations. Decreases in the expression levels of the antiapoptotic proteins c‐FLIP and XIAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:739–745, 2010 |
| doi_str_mv | 10.1002/jor.21056 |
| format | article |
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Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL‐induced Bid and caspase‐3 activations. Decreases in the expression levels of the antiapoptotic proteins c‐FLIP and XIAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:739–745, 2010</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.21056</identifier><identifier>PMID: 20041491</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>apoptosis ; Apoptosis - drug effects ; Bone Neoplasms - metabolism ; Bone Neoplasms - pathology ; Bone Neoplasms - radiotherapy ; Cell Line, Tumor ; Cell Survival - drug effects ; Cell Survival - radiation effects ; DR5 ; Gene Expression Regulation, Neoplastic - radiation effects ; Humans ; ionizing radiation ; Osteosarcoma - metabolism ; Osteosarcoma - pathology ; Osteosarcoma - radiotherapy ; Promoter Regions, Genetic ; Receptors, TNF-Related Apoptosis-Inducing Ligand - genetics ; Receptors, Tumor Necrosis Factor - genetics ; RNA, Messenger - analysis ; TNF-Related Apoptosis-Inducing Ligand - pharmacology ; TRAIL ; Up-Regulation</subject><ispartof>Journal of orthopaedic research, 2010-06, Vol.28 (6), p.739-745</ispartof><rights>Copyright © 2009 Orthopaedic Research Society</rights><rights>(c) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4636-a602e07b6968c7821ca0944e5e193c8fa45539976540c2f4a021eba2ad61ae173</citedby><cites>FETCH-LOGICAL-c4636-a602e07b6968c7821ca0944e5e193c8fa45539976540c2f4a021eba2ad61ae173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20041491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hori, Takeshi</creatorcontrib><creatorcontrib>Kondo, Takashi</creatorcontrib><creatorcontrib>Kanamori, Masahiko</creatorcontrib><creatorcontrib>Tabuchi, Yoshiaki</creatorcontrib><creatorcontrib>Ogawa, Ryohei</creatorcontrib><creatorcontrib>Zhao, Qing-Li</creatorcontrib><creatorcontrib>Ahmed, Kanwal</creatorcontrib><creatorcontrib>Yasuda, Taketoshi</creatorcontrib><creatorcontrib>Seki, Shoji</creatorcontrib><creatorcontrib>Suzuki, Kayo</creatorcontrib><creatorcontrib>Kimura, Tomoatsu</creatorcontrib><title>Ionizing radiation enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulations of death receptor 4 (DR4) and death receptor 5 (DR5) in human osteosarcoma cells</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL‐induced Bid and caspase‐3 activations. Decreases in the expression levels of the antiapoptotic proteins c‐FLIP and XIAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:739–745, 2010</description><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Bone Neoplasms - metabolism</subject><subject>Bone Neoplasms - pathology</subject><subject>Bone Neoplasms - radiotherapy</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>DR5</subject><subject>Gene Expression Regulation, Neoplastic - radiation effects</subject><subject>Humans</subject><subject>ionizing radiation</subject><subject>Osteosarcoma - metabolism</subject><subject>Osteosarcoma - pathology</subject><subject>Osteosarcoma - radiotherapy</subject><subject>Promoter Regions, Genetic</subject><subject>Receptors, TNF-Related Apoptosis-Inducing Ligand - genetics</subject><subject>Receptors, Tumor Necrosis Factor - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>TNF-Related Apoptosis-Inducing Ligand - pharmacology</subject><subject>TRAIL</subject><subject>Up-Regulation</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp1kV1v0zAUhiMEYmVwwR9AvmO9yGY7_mgupwKjU7WhamjcWafOSeuR2J2dCMb_5P-QrNuEkLiy5POcx9b7ZtlbRo8ZpfzkJsRjzqhUz7IJk1Lkkutvz7MJ1YXKKVfqIHuV0g2lVDM-e5kdcEoFEyWbZL8Xwbtfzm9IhMpB54In6LfgLSbS9W2IxKONIblEarBdiHnEBjqsCOzCrhsHufNVb0dH4zbgK3J0tTpdLKf7-79J0m1j6Ddb0u8GzaZv7h9MJNSkQui2JKLFAY1EkKMPKzElo-6fkRxHckqcJ9u-BU9C6jAkiDa0QCw2TXqdvaihSfjm4TzMvn76eDX_nC8vzxbz02VuhRqiAUU5Ur1WpZpZPePMAi2FQImsLOysBiFlUZZaSUEtrwVQznANHCrFAJkuDrP3e-8uhtseU2dal8YfgMfQJ6OLQpZcaTqQ0z05Zpki1mYXXQvxzjBqxhLNUKK5L3Fg3z1Y-3WL1RP52NoAnOyBH67Bu_-bzPnl6lGZ7zfckNXPpw2I343ShZbm-uLMfOGri3M11-a6-ANQC7hB</recordid><startdate>201006</startdate><enddate>201006</enddate><creator>Hori, Takeshi</creator><creator>Kondo, Takashi</creator><creator>Kanamori, Masahiko</creator><creator>Tabuchi, Yoshiaki</creator><creator>Ogawa, Ryohei</creator><creator>Zhao, Qing-Li</creator><creator>Ahmed, Kanwal</creator><creator>Yasuda, Taketoshi</creator><creator>Seki, Shoji</creator><creator>Suzuki, Kayo</creator><creator>Kimura, Tomoatsu</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201006</creationdate><title>Ionizing radiation enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulations of death receptor 4 (DR4) and death receptor 5 (DR5) in human osteosarcoma cells</title><author>Hori, Takeshi ; Kondo, Takashi ; Kanamori, Masahiko ; Tabuchi, Yoshiaki ; Ogawa, Ryohei ; Zhao, Qing-Li ; Ahmed, Kanwal ; Yasuda, Taketoshi ; Seki, Shoji ; Suzuki, Kayo ; Kimura, Tomoatsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4636-a602e07b6968c7821ca0944e5e193c8fa45539976540c2f4a021eba2ad61ae173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Bone Neoplasms - metabolism</topic><topic>Bone Neoplasms - pathology</topic><topic>Bone Neoplasms - radiotherapy</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>DR5</topic><topic>Gene Expression Regulation, Neoplastic - radiation effects</topic><topic>Humans</topic><topic>ionizing radiation</topic><topic>Osteosarcoma - metabolism</topic><topic>Osteosarcoma - pathology</topic><topic>Osteosarcoma - radiotherapy</topic><topic>Promoter Regions, Genetic</topic><topic>Receptors, TNF-Related Apoptosis-Inducing Ligand - genetics</topic><topic>Receptors, Tumor Necrosis Factor - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>TNF-Related Apoptosis-Inducing Ligand - pharmacology</topic><topic>TRAIL</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hori, Takeshi</creatorcontrib><creatorcontrib>Kondo, Takashi</creatorcontrib><creatorcontrib>Kanamori, Masahiko</creatorcontrib><creatorcontrib>Tabuchi, Yoshiaki</creatorcontrib><creatorcontrib>Ogawa, Ryohei</creatorcontrib><creatorcontrib>Zhao, Qing-Li</creatorcontrib><creatorcontrib>Ahmed, Kanwal</creatorcontrib><creatorcontrib>Yasuda, Taketoshi</creatorcontrib><creatorcontrib>Seki, Shoji</creatorcontrib><creatorcontrib>Suzuki, Kayo</creatorcontrib><creatorcontrib>Kimura, Tomoatsu</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hori, Takeshi</au><au>Kondo, Takashi</au><au>Kanamori, Masahiko</au><au>Tabuchi, Yoshiaki</au><au>Ogawa, Ryohei</au><au>Zhao, Qing-Li</au><au>Ahmed, Kanwal</au><au>Yasuda, Taketoshi</au><au>Seki, Shoji</au><au>Suzuki, Kayo</au><au>Kimura, Tomoatsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ionizing radiation enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulations of death receptor 4 (DR4) and death receptor 5 (DR5) in human osteosarcoma cells</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. 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| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | apoptosis Apoptosis - drug effects Bone Neoplasms - metabolism Bone Neoplasms - pathology Bone Neoplasms - radiotherapy Cell Line, Tumor Cell Survival - drug effects Cell Survival - radiation effects DR5 Gene Expression Regulation, Neoplastic - radiation effects Humans ionizing radiation Osteosarcoma - metabolism Osteosarcoma - pathology Osteosarcoma - radiotherapy Promoter Regions, Genetic Receptors, TNF-Related Apoptosis-Inducing Ligand - genetics Receptors, Tumor Necrosis Factor - genetics RNA, Messenger - analysis TNF-Related Apoptosis-Inducing Ligand - pharmacology TRAIL Up-Regulation |
| title | Ionizing radiation enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulations of death receptor 4 (DR4) and death receptor 5 (DR5) in human osteosarcoma cells |
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