Effects of a prolonged standardized diet on normalizing the human metabolome

BACKGROUND: Although the effects of acute dietary interventions on the human metabolome have been studied, the extent to which the metabolome can be normalized by extended dietary standardization has not yet been examined. OBJECTIVE: We examined the metabolic profiles of healthy human subjects after...

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Bibliographic Details
Published in:The American journal of clinical nutrition 2009-12, Vol.90 (6), p.1496-1501
Main Authors: Winnike, Jason H, Busby, Marjorie G, Watkins, Paul B, O'Connell, Thomas M
Format: Article
Language:English
Subjects:
Adolescent
Adult
age
Biological and medical sciences
cohort studies
Diet
diet therapy
Feeding. Feeding behavior
Female
food intake
functional foods
Fundamental and applied biological sciences. Psychology
genetics
health effects assessments
health status
human health
Humans
lifestyle
long term effects
Magnetic Resonance Spectroscopy
Male
Metabolism
metabolites
Metabolome
Middle Aged
Multivariate analysis
NMR
Nuclear magnetic resonance
Nutrition
nutritional intervention
Principal Component Analysis
sociodemographic characteristics
Spectrum analysis
Urinalysis
urine
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:BACKGROUND: Although the effects of acute dietary interventions on the human metabolome have been studied, the extent to which the metabolome can be normalized by extended dietary standardization has not yet been examined. OBJECTIVE: We examined the metabolic profiles of healthy human subjects after extended dietary standardization to see whether the inherent variation in the human metabolome could be decreased. DESIGN: A cohort of 10 healthy volunteers was admitted to a clinical research center for 2 wk of dietary standardization. Daily serum and urine samples and serum samples at a 2-wk follow-up visit were collected. The samples were analyzed by ¹H nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analyses. RESULTS: NMR spectra were collected to globally profile the higher-concentration metabolites (>μmol/L concentrations). Metabolic changes were observed in some serum samples after day 1 or the 2-wk follow-up visit. For each subject, the samples from all other days had similar profiles. The urinary metabolome reflected no effects from dietary standardization. Pooled 24-h urine samples were studied, which indicated that any normalization that does occur would do so in
ISSN:0002-9165
1938-3207
DOI:10.3945/ajcn.2009.28234