SEE Locomotor Behavior Test Discriminates C57BL/6J and DBA/2J Mouse Inbred Strains Across Laboratories and Protocol Conditions

Conventional tests of behavioral phenotyping frequently have difficulties differentiating certain genotypes and replicating these differences across laboratories and protocol conditions. This study explores the hypothesis that automated tests can be designed to quantify ethologically relevant behavi...

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Bibliographic Details
Published in:Behavioral neuroscience 2003-06, Vol.117 (3), p.464-477
Main Authors: Kafkafi, Neri, Lipkind, Dina, Benjamini, Yoav, Mayo, Cheryl L, Elmer, Gregory I, Golani, Ilan
Format: Article
Language:English
Subjects:
Activity Level
Animal
Animal ethology
Animal Strain Differences
Animals
Behavioral Genetics
Biological and medical sciences
Experimental Laboratories
Experimental Methods
Exploratory Behavior - physiology
Fundamental and applied biological sciences. Psychology
General aspects
Genotype
Genotypes
Laboratories - standards
Laboratories - statistics & numerical data
Male
Mice
Mice, Inbred C57BL - physiology
Mice, Inbred DBA - physiology
Motor Activity - physiology
Phenotypes
Psychology. Psychoanalysis. Psychiatry
Research Design - standards
Research Design - statistics & numerical data
Species Specificity
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Summary:Conventional tests of behavioral phenotyping frequently have difficulties differentiating certain genotypes and replicating these differences across laboratories and protocol conditions. This study explores the hypothesis that automated tests can be designed to quantify ethologically relevant behavior patterns that more readily characterize heritable and replicable phenotypes. It used SEE (Strategy for the Exploration of Exploration) to phenotype the locomotor behavior of the C57BL/6 and DBA/2 mouse inbred strains across 3 laboratories. The 2 genotypes differed in 15 different measures of behavior, none of which had a significant genotype-laboratory interaction. Within the same laboratory, most of these differences were replicated in additional experiments despite the test photoperiod phase being changed and saline being injected. Results suggest that well-designed tests may considerably enhance replicability across laboratories.
ISSN:0735-7044
1939-0084
DOI:10.1037/0735-7044.117.3.464