The Role of Translationally Controlled Tumor Protein in Tumor Growth and Metastasis of Colon Adenocarcinoma Cells

Translationally controlled tumor protein (TCTP) plays a major role in a broad array of biological processes. However, the TCTP-related biological process and interactive proteins still remain poorly characterized. In the present study, we found that knockdown of TCTP inhibited proliferation, migrati...

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Bibliographic Details
Published in:Journal of proteome research 2010-01, Vol.9 (1), p.40-49
Main Authors: Ma, Qiang, Geng, Yan, Xu, Weiwen, Wu, Yingsong, He, Fuli, Shu, Wen, Huang, Maoliang, Du, Hongyan, Li, Ming
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Animals
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Biomarkers, Tumor - physiology
Cell Adhesion - genetics
Cell Growth Processes - genetics
Cell Line, Tumor
Cell Movement - genetics
Colonic Neoplasms - genetics
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Gene Knockdown Techniques
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Metastasis
Protein Biosynthesis
Proteomics - methods
Reverse Transcriptase Polymerase Chain Reaction
RNA, Small Interfering - administration & dosage
RNA, Small Interfering - genetics
Xenograft Model Antitumor Assays
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Summary:Translationally controlled tumor protein (TCTP) plays a major role in a broad array of biological processes. However, the TCTP-related biological process and interactive proteins still remain poorly characterized. In the present study, we found that knockdown of TCTP inhibited proliferation, migration, and invasion activities of LoVo cells in vitro and in vivo. The whole-cell proteomes were compared by 2D gel electrophoresis before and after knockdown of TCTP. Alterations in 27 proteins were detected and their identities were revealed by mass spectrometry analysis. Components of Ubiquitin-Proteasome System, proteins involved in the cytoskeleton biosynthesis and tumor metastasis were found to be changed upon TCTP removal. These results imply that TCTP might play at least a partial role in colon adenocarcinoma progression.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr9001367