Characterization in vivo and in vitro of a strain of Leishmania (Viannia) shawi from the Amazon Region

Abstract The present study analyses complement resistance, cell surface carbohydrates expression, lipidic composition and morphology in vivo and in vitro , of Leishmania (Viannia) shawi , a parasite identified in the Amazon region, Pará state, in 1989. We demonstrated that promastigotes in the stati...

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Bibliographic Details
Published in:Parasitology international 2009-06, Vol.58 (2), p.154-160
Main Authors: Ramos, Patrícia K.S, Diniz, José A.P, Silva, Edilene O, Quaresma, Juarez A.S, Saraiva, Elvira M, Seabra, Sérgio H, Atella, Geórgia C, de Souza, Wanderley
Format: Article
Language:English
Subjects:
Amazon Region
Animals
Brazil
Carbohydrate
Carbohydrates - analysis
Carbohydrates - chemistry
Complement resistance
Complement System Proteins - immunology
Cricetinae
Gastroenterology and Hepatology
Guinea Pigs
Humans
Infectious Disease
Lectins - metabolism
Leishmania
Leishmania (Viannia) shawi
Leishmania - classification
Leishmania - growth & development
Leishmania - pathogenicity
Leishmania - ultrastructure
Leishmaniasis, Cutaneous - immunology
Leishmaniasis, Cutaneous - parasitology
Leishmaniasis, Cutaneous - pathology
Mesocricetus
Microscopy - instrumentation
Microscopy, Electron
Susceptibility
Ultrastructure
Viannia
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Summary:Abstract The present study analyses complement resistance, cell surface carbohydrates expression, lipidic composition and morphology in vivo and in vitro , of Leishmania (Viannia) shawi , a parasite identified in the Amazon region, Pará state, in 1989. We demonstrated that promastigotes in the stationary (STAT) growth phase are more resistant to complement lysis than in the logarithimic (LOG) growth phase. Ultrastructural analyses and imidazol technique showed accumulation of lipids in STAT growth phase promastigotes, which was confirmed by biochemical approach. Light and electron microscopy of skin lesion in hamster footpads caused by promastigotes in STAT growth phase, 90 days post inoculation, showed amastigotes inside of macrophage and free in the tissue surrounded by collagen fibers as well as extensive inflammatory reaction with tissue destruction. We also demonstrated, using lectins by agglutination assays and flow cytometry, the presence of fucose, mannose and/or glucose carbohydrate residues on the surface of LOG and STAT promastigotes. The results constitute the first characterization essay combining biochemical and morphological approaches dedicated to LOG and STAT growth phase promastigotes of L. (V) shawi contributing for a better knowledge of this poorly studied species of the New World.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2009.01.009