Immunogenicity of recombinant Mannheimia haemolytica serotype 1 outer membrane protein PlpE and augmentation of a commercial vaccine

Mannheimia haemolytica is the major cause of severe bacterial pneumonia associated with shipping fever in cattle. The gene for M. haemolytica outer membrane protein (OMP) PlpE was cloned into the expression vector pRSETA. The cloned gene was then expressed in BL21(DE3)pLysS and the recombinant PlpE...

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Bibliographic Details
Published in:Vaccine 2003-06, Vol.21 (21), p.2821-2829
Main Authors: Confer, Anthony W., Ayalew, Sahlu, Panciera, Roger J., Montelongo, Marie, Whitworth, Lisa C., Hammer, Jordan D.
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Bacterial Proteins - immunology
Bacterial Vaccines - immunology
Bacteriology
Cattle
Cattle Diseases - immunology
Cattle Diseases - prevention & control
Cattle pneumonia
Immunogenicity
Injections, Subcutaneous
Mannheimia haemolytica
Mannheimia haemolytica - immunology
Medical research
Outer membrane protein
Pasteurellaceae Infections - immunology
Pasteurellaceae Infections - prevention & control
Pasteurellaceae Infections - veterinary
PlpE
Serotyping
Studies
Time Factors
Vaccination
Vaccination - veterinary
Vaccines
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Summary:Mannheimia haemolytica is the major cause of severe bacterial pneumonia associated with shipping fever in cattle. The gene for M. haemolytica outer membrane protein (OMP) PlpE was cloned into the expression vector pRSETA. The cloned gene was then expressed in BL21(DE3)pLysS and the recombinant PlpE (rPlpE) was purified and used in immunological and vaccination studies. Vaccination of cattle with commercial M. haemolytica vaccines stimulated no significant ( P>0.05) antibody responses to rPlpE. Recombinant PlpE in a commercial proprietary adjuvant was highly immunogenic when injected subcutaneously into cattle. Vaccination of cattle with 100 μg of rPlpE markedly enhanced resistance against experimental challenge with virulent M. haemolytica. Addition of 100 μg of rPlpE to a commercial M. haemolytica vaccine, Presponse ®, significantly enhanced ( P
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00213-5