Regulation of lac operon expression in mixed sugar chemostat cultures

Expression of the lactose (lac) operon in the Escherichia coli chromosome has been studied in mixed‐sugar chemostat cultures under steady‐state and transient conditions. A unified model has been formulated which involves regulation of active inducer (lactose) transport, promoter–operator regulated e...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1987-06, Vol.29 (8), p.1003-1014
Main Authors: Ray, N. G., Vieth, W. R., Venkatasubramanian, K.
Format: Article
Language:English
Subjects:
Applied microbiology
Biological and medical sciences
Biotechnology
carbon sources
chemostats
Escherichia coli
Fundamental and applied biological sciences. Psychology
gene regulation
General aspects
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
operons
sugars
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Summary:Expression of the lactose (lac) operon in the Escherichia coli chromosome has been studied in mixed‐sugar chemostat cultures under steady‐state and transient conditions. A unified model has been formulated which involves regulation of active inducer (lactose) transport, promoter–operator regulated expression of the lac operon, glucose‐mediated inducer exclusion, and catabolite repression. The model of the lac operon control system focuses on the molecular interactions among the regulatory species and the genetic control elements for the initiation of transcription. The role of catabolite modulator factor (CMF) in the regulation of transcription is described. The modeling of glucose‐mediated regulation of intracellular cyclic adenosine monophosphate (cAMP) and inducer exclusion is based on the recently elucidated mechanisms of the involvement of the PTS (phosphoen‐olpyruvate dependent sugar transport system) enzymes, in the presence of glucose, in regulation of adenylate cyclase and non‐PTS sugar transport proteins (i.e. per‐meases). The adequacy of the unified model was verified with experimental data.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260290812