Modulation of KB and A431 cell adhesion by epidermal growth inhibitory pentapeptide

A pentapaptide, pyroGlu-Glu-Asp-Ser-GlyOH (EPP), isolated from mouse epidermis, inhibits mitoses and enhances differentiation in primary cultures and in transformed mouse epidermal cells in vitro (Elgjo et al. 1986 b). The present work demonstrates that EPP also modulates the adhesiveness of two hum...

Full description

Saved in:
Bibliographic Details
Published in:Virchows Archiv. Abteilung B. Zellpathologie 1992-12, Vol.62 (6), p.353-356
Main Authors: Ushmorov, A A, Danilov, A O, Zubova, S G, Elgjo, K, Okulov, V B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Cell Adhesion - drug effects
Collagen
Fibrinogen
Growth Inhibitors - pharmacology
Humans
Molecular Sequence Data
Oligopeptides - pharmacology
Plastics
Pyrrolidonecarboxylic Acid - analogs & derivatives
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A pentapaptide, pyroGlu-Glu-Asp-Ser-GlyOH (EPP), isolated from mouse epidermis, inhibits mitoses and enhances differentiation in primary cultures and in transformed mouse epidermal cells in vitro (Elgjo et al. 1986 b). The present work demonstrates that EPP also modulates the adhesiveness of two human tumour cell lines (KB and A431) of epidermal origin to uncoated plastic and to plastic coated with fibrinogen or collagen type 1. The adhesion modulatory effect of EPP was observed over a broad range of concentrations (10(-12)-10(-6) M), and depended on the substrate the cells were growing on. Thus, when cells were seeded on plastic or collagen, the attachment to the substrate was suppressed at the highest concentrations of EPP, and stimulated at the lowest ones. The opposite concentration-response pattern was observed when fibrinogen was used as substrate.
ISSN:0340-6075
0042-6431
DOI:10.1007/BF02899703