Paracoccidioides brasiliensis uses endogenous and exogenous arachidonic acid for PGE x production

Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent deep mycosis in Latin America. Production of eicosanoids during fungal infections plays a critical role on fungal biology as well as on host immune response modulation. The purpose of our study was to assess whe...

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Bibliographic Details
Published in:Mycopathologia (1975) 2010-08, Vol.170 (2), p.123-130
Main Authors: Biondo, Guilherme Augusto, Dias-Melicio, Luciane Alarcão, Bordon-Graciani, Ana Paula, Acorci-Valério, Michele Janegitz, Soares, Angela Maria Victoriano Campos
Format: Article
Language:English
Subjects:
Antifungal Agents - metabolism
Arachidonic Acid - metabolism
Culture Media - chemistry
Fungal Proteins - metabolism
Indomethacin - metabolism
Microbial Viability
Paracoccidioides - metabolism
Paracoccidioides - pathogenicity
Prostaglandin-Endoperoxide Synthases - metabolism
Prostaglandins E - biosynthesis
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Summary:Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent deep mycosis in Latin America. Production of eicosanoids during fungal infections plays a critical role on fungal biology as well as on host immune response modulation. The purpose of our study was to assess whether P. brasiliensis strains with different degree of virulence (Pb18, Pb265, Bt79, Pb192) produce prostaglandin E(x) (PGE(x)). Moreover, we asked if P. brasiliensis could use exogenous sources of arachidonic acid (AA), as well as metabolic pathways dependent on cyclooxygenase (COX) enzyme, as reported for mammalian cells. A possible association between this prostanoid and fungus viability was also assessed. Our results showed that all strains, independently of their virulence, produce high PGE(x) levels on 4 h culture that were reduced after 8 h. However, in both culture times, higher prostanoid levels were detected after supplementation of medium with exogenous AA. Treatment with indomethacin, a COX inhibitor, induced a reduction on PGEx, as well as in fungus viability. The data provide evidence that P. brasiliensis produces prostaglandin-like molecules by metabolizing either endogenous or exogenous AA. Moreover, the results suggest the involvement of these mediators on fungal viability.
ISSN:1573-0832
DOI:10.1007/s11046-010-9301-x