Induction of micronuclei and nuclear abnormalities by cyclophosphamide and colchicine in Xenotoca melanosoma (Pisces, Goodeidae) from Lake La Alberca in Michoacán, México

• Published in: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering Toxic/hazardous substances & environmental engineering, 2010-01, Vol.45 (1), p.75-81
• Main Authors: Zavala-Aguirre, José Luis, Torres-Bugarín, Olivia, Buelna-Osben, Héctor René, Flores-Kehn, Lola Paulina, Ramos-Ibarra, María Luisa, Zúñiga-González, Guillermo, Ogura, Tetsuya
• Format: Article
• Language: English
• Subjects: Abnormalities; Aneugens - toxicity; Animals; Applied sciences; Bioassays; bioindicator; Blood; Cell Nucleus - drug effects; Cell Nucleus - pathology; Colchicine; Colchicine - toxicity; Continental surface waters; cyclophosphamide; Cyclophosphamide - toxicity; Cyprinodontiformes; Dose-Response Relationship, Drug; Ecosystems; Environmental engineering; Environmental Monitoring; Erythroblasts - drug effects; Erythroblasts - physiology; Erythrocytes; Exact sciences and technology; Female; Fresh Water; Goodeidae; Hazardous; Health; Lakes; Male; Mexico; Micronuclei, Chromosome-Defective - chemically induced; Micronucleus Tests; Mutagens - toxicity; Natural water pollution; nuclear abnormalities; Organisms; peripheral blood erythrocytes; Pisces; Pollution; spontaneous micronuclei; Studies; Toxic; Toxicology; Water treatment and pollution; Watersheds; Xenotoca melanosoma
• ISSN: 1093-4529; 1532-4117
• DOI: 10.1080/10934520903388863

This study is a follow-up of previous research in which we described the frequency of spontaneous micronucleated erythrocytes (MNE) in the Goodeid Xenotocoa melanosoma collected from Lake La Alberca, located in the state of Michoacán, México. In the present work, we measured micronuclei (MN) and nuclear abnormalities (NA) in erythrocytes of peripheral blood. Bioassays taken at 24 or 96 hours in either the cyclophosfamide (CP) or colchicine (COL) showed a significant increase in MN and BC (P values ranging from 0.0499 to 0.0036) compared with information from wild organisms collected over 3 years. Concentrationdependent and time-dependent responses support the proposal of using endemic Xenotoca melanosoma as a bioindicator of genotoxicity and cytotoxicity with a high transcendence for the health of the entire ecosystem and evaluation of the Lerma-Chapala watershed.