Isolation of bovine herpesvirus type 5 from the semen of a healthy bull in Australia

Artificial insemination is widely used in the cattle industry and a major challenge is to ensure that semen is free of infectious agents. A healthy donor bull was tested for freedom from infectious agents. A bovine herpesvirus was isolated in testis cells and identified as bovine herpesvirus type 5...

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Bibliographic Details
Published in:Australian veterinary journal 2010-03, Vol.88 (3), p.93-95
Main Authors: Diallo, I.S, Hewitson, G.R, Hoad, J, Turner, S, Corney, B.G, Rodwell, B.J
Format: Article
Language:English
Subjects:
Animals
artificial insemination
Australia
bovine herpesvirus 1 (BoHV-1)
Bovine herpesvirus 5
bovine herpesvirus 5 (BoHV-5)
bulls
Cattle
cattle diseases
Cattle Diseases - epidemiology
Cattle Diseases - transmission
Cattle Diseases - virology
cattle reproduction
disease transmission
DNA, Viral - chemistry
DNA, Viral - genetics
Encephalitis, Viral - epidemiology
Encephalitis, Viral - transmission
Encephalitis, Viral - veterinary
Encephalitis, Viral - virology
Herpesviridae Infections - epidemiology
Herpesviridae Infections - transmission
Herpesviridae Infections - veterinary
Herpesviridae Infections - virology
Herpesvirus 5, Bovine - isolation & purification
Insemination, Artificial - veterinary
Male
Meningoencephalitis - epidemiology
Meningoencephalitis - transmission
Meningoencephalitis - veterinary
Meningoencephalitis - virology
microbial contamination
molecular sequence data
molecular virology
new geographic records
nucleotide sequences
pathogen identification
polymerase chain reaction
Polymerase Chain Reaction - veterinary
semen
Semen - virology
viral diseases of animals and humans
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Summary:Artificial insemination is widely used in the cattle industry and a major challenge is to ensure that semen is free of infectious agents. A healthy donor bull was tested for freedom from infectious agents. A bovine herpesvirus was isolated in testis cells and identified as bovine herpesvirus type 5 (BoHV‐5) by polymerase chain reaction and by direct amplicon sequencing. The amplicon sequence shared 100% similarity with the published sequence of BoHV‐5. This is the first report in Australia of BoHV‐5 in semen. The implications of this finding are discussed.
ISSN:0005-0423
1751-0813
DOI:10.1111/j.1751-0813.2009.00545.x