Analysis of acquired resistance genes in Stenotrophomonas maltophilia

• Published in: Taehan Chindan Kŏmsa Ŭihakhoe chi 2010-06, Vol.30 (3), p.295-300
• Main Authors: Song, Jeong Hoon, Sung, Ji Youn, Kwon, Kye Chul, Park, Jong Woo, Cho, Hye Hyun, Shin, So Yeon, Ko, Young Hyun, Kim, Ji Myung, Shin, Kyeong Seob, Koo, Sun Hoe
• Format: Article
• Language: Korean
• Subjects: Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; Carrier Proteins - genetics; Disk Diffusion Antimicrobial Tests; DNA, Bacterial - genetics; Drug Resistance, Multiple, Bacterial - genetics; Humans; Integrons - genetics; Polymerase Chain Reaction; Stenotrophomonas maltophilia - drug effects; Stenotrophomonas maltophilia - genetics; Stenotrophomonas maltophilia - isolation & purification; Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology
• ISSN: 1598-6535
• DOI: 10.3343/kjlm.2010.30.3.295

Stenotrophomonas maltophilia is a gram-negative bacillus and a nosocomial pathogen in immunocompromised patients. Trimethoprim/sulfamethoxazole (TMP/SMX) is the drug of choice for treating S. maltophilia infection; however, resistance to TMP/SMX is increasing. In this study, we investigated the relationship between the incidence of TMP/SMX resistance and the presence of sul genes and mobile elements. A total of 120 S. maltophilia isolates were collected from 3 university hospitals between April 2007 and April 2009. Antimicrobial susceptibilities were determined using the disk diffusion method. PCR and DNA sequencing were conducted for the detection of sul1, sul2, class 1 integron, and ISCR2 element. Repetitive extragenic palindromic sequence-based PCR (REP-PCR) was carried out to evaluate the genetic relatedness. The TMP/SMX-resistant (R) isolates harbored a significantly higher proportion of sul1 gene and class 1 integron than TMP/SMX-susceptible (S) isolates (P