Encapsulation of nodal cuttings and shoot tips for storage and exchange of cassava germplasm

We report the encapsulation of in vitro-derived nodal cuttings or shoot tips of cassava in 3% calcium alginate for storage and germplasm exchange purposes. Shoot regrowth was not significantly affected by the concentration of sucrose in the alginate matrix while root formation was. In contrast, incr...

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Bibliographic Details
Published in:Plant cell reports 2003-04, Vol.21 (8), p.718-725
Main Authors: DANSO, K. E, FORD-LLOYD, B. V
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - pharmacology
Alginates - pharmacology
Benzyl Compounds
Biological and medical sciences
Calcium chloride
Cuttings
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Genetic resources
Glucuronic Acid
Hexuronic Acids
Kinetin
Manihot - drug effects
Manihot - genetics
Manihot - growth & development
Meristem - drug effects
Meristem - growth & development
Naphthaleneacetic Acids - pharmacology
Plant Growth Regulators - pharmacology
Plant Roots - drug effects
Plant Roots - growth & development
Plant Shoots - drug effects
Plant Shoots - growth & development
Plant Stems - drug effects
Plant Stems - growth & development
Purines
Regrowth
Sucrose - pharmacology
Time Factors
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Summary:We report the encapsulation of in vitro-derived nodal cuttings or shoot tips of cassava in 3% calcium alginate for storage and germplasm exchange purposes. Shoot regrowth was not significantly affected by the concentration of sucrose in the alginate matrix while root formation was. In contrast, increasing the sucrose concentration in the calcium chloride polymerisation medium significantly reduced regrowth from encapsulated nodal cuttings of accession TME 60444. Supplementing the alginate matrix with increased concentrations of 6-benzylaminopurine and alpha-naphthaleneacetic acid enhanced complete plant regrowth within 2 weeks. Furthermore, plant regrowth by encapsulated nodal cuttings and shoot tips was significantly affected by the duration of the storage period as shoot recovery decreased from almost 100% to 73.3% for encapsulated nodal cuttings and 94.4% to 60% for shoot tips after 28 days of storage. The high frequency of plant regrowth from alginate-coated micropropagules coupled with high viability percentage after 28 days of storage is highly encouraging for the exchange of cassava genetic resources. Such encapsulated micropropagules could be used as an alternative to synthetic seeds derived from somatic embryos.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-003-0594-9