Macroporous and hydrophilic polymer resins modified with isothiocyanate groups for immobilization of enzymes

Hydrophilic and macroporous polymer resins composed of glycerylmethacrylate, styrene, and divinylbenzene were quite easily modified with isothiocyanate groups using a Friedel–Crafts reaction with 3‐chloropropionyl chloride and subsequent nucleophilic reaction with KSCN. Alkaline phosphatase and tryp...

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Published in:Biotechnology and bioengineering 1990-07, Vol.36 (3), p.219-223
Main Authors: Hasegawa, Masanori, Kitano, Hiromi, Nishida, Ryosuke, Kobashi, Toshiyuki
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
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creator Hasegawa, Masanori
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Kobashi, Toshiyuki
description Hydrophilic and macroporous polymer resins composed of glycerylmethacrylate, styrene, and divinylbenzene were quite easily modified with isothiocyanate groups using a Friedel–Crafts reaction with 3‐chloropropionyl chloride and subsequent nucleophilic reaction with KSCN. Alkaline phosphatase and trypsin could be covalently bound to the isothiocyanate‐carrying polymer resins, and the immobilized enzymes obtained were sufficiently active and stable due to a covalent bonding via a spacer group between the carrier resins and the enzymes and also due to a hydrophilic environment around the enzymes. A heterogeneity of the immobilized enzyme was taken into account to interpret the thermal denaturation process of the enzyme immobilized onto the resins.
doi_str_mv 10.1002/bit.260360302
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subjects Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
title Macroporous and hydrophilic polymer resins modified with isothiocyanate groups for immobilization of enzymes
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